Bacterial species and genera associated with subsequent development of aGVHD. (A-B) The relative abundance (% on a –log₁₀ scale) of the significant predefined bacteria, Blautia and A muciniphila, respectively, among those who do not develop aGVHD vs those who do develop aGVHD early post-aHSCT. (C-D) Our discovery method for bacterial genera and species (present in ≥10% of samples tested at a minimum of 0.01% relative abundance, colored by bacterial order) in early post-aHSCT samples. Those under the dotted line have not passed the DESeq2 threshold of an FDR of P < .01. Those depicted by a larger dot have a lower P value from the Wilcoxon test taking into account compositionality. Those with the largest dot (P ≤ .05) and above the dotted line are the significant bacteria based on our discovery model. The only bacterial genera that have passed this are Blautia, Campylobacter, and Akkermansia, which are shown as the labeled dot on the top of the graph and for species A muciniphila. To see all bacteria passing either threshold (DESeq or Wilcoxon), see supplemental Figure 4.