CD3-LVs transduce human T cells in whole blood. CD3-LVs and VSV-LV were tested for their ability to transduce T cells in whole blood of healthy donors in absence of additional stimuli and transduction enhancer. (A) Experimental procedure. (B) Representative dot plots show the percentages of GFP⁺ cells gated from all viable single cells at day 5 posttransduction in isolated PBMC. (C-D) Bar diagrams show the percentages of GFP⁺ cells of all viable single cells (C) and in CD4⁺ and CD8⁺ cell subsets (D) at day 5 posttranduction. *P < .05, **P < .01 by mixed-effect analysis with Sidak's correction. Mean ± SD of 3 independent experiments with 5 different donors. *P < .05, **P < .01 by mixed-effect analysis with Dunnett's correction.