Detailed genetic analyses of the family members. (A-B) Copy-number analysis of the TM2D3 gene using the QuantStudio 3D Digital PCR system. Non-PNH cells from II-7 had heterozygous deletions, and PNH cells from II-7 and III-1 had homozygous deletions, whereas other family members showed normal copy number. Experiments shown in panels A and B were performed at the different time points using the same samples in case of III-1 and control. Standard deviation bars are shown from the independent triplicate analysis.