Genome-scale CRISPR-Cas9 knockout screening identifies STAT3 as a repressor of CD38. (A) Flowchart of genome-scale CRIPSR-Cas9 screening in RPMI 8226 cells. (B) List of top 10 positive (left panel) and negative (right panel) ranked genes; positive represents sgRNAs enriched in sorted cells compared with control cells, and negative the opposite. STAT3, JAK1, and CD38 are highlighted in yellow. (C) Enrichment of specific sgRNAs in CD38-high fraction. Each dot specifies 1 sgRNA. (D-F) Read counts of sgRNAs against STAT3 (D), JAK1 (E), and CD38 (F) in control and sorted cells.