Reversal of a venous thrombosis defect and afibrinogenemia with fibrinogen AαE, but not Aα, expression in early zebrafish larvae. (A) One- to 2-cell embryos from an fga^+/− in cross mating were microinjected with reagents for transgenic expression of fibrinogen Aα or AαE cDNAs under the control of a ubb (ubi P) promoter sequence. At 3 dpf, TTO was measured after laser injury of the PCV as illustrated. (B) Results are shown with the fga genotype, number of larvae used in brackets, and, where appropriate, cDNA expressed on the y-axis. Each shape represents an individual larva. Mann-Whitney U tests were made to test for statistical significance of differences between groups (****P < .0001).