PRT-treated platelets induce TGF-β production in CD4⁺cDCs. The responses of cDCs were examined at 4 hours (day 0) and at days 1, 2, and 6. (A) Cells were gated on CD11c^Hi CD8⁺, CD11c^Hi CD8⁻ CD11b⁺ CD4⁺ or CD11c^Hi CD8⁻ CD11b⁺ CD4⁻ cDC subsets. (B) The frequencies of CD86⁺ and TGF-β⁺ cells and the median fluorescent intensities of CD80 and MHCII were evaluated for each cDC subset. (C-E) Heat maps show fold changes of each group compared with the nontransfused group. The frequency of total cDCs was derived from total live-cell events, and the frequency of CD11c^Hi CD8⁺ (C), CD11c^Hi CD8⁻ CD11b⁺ CD4⁺ (D), and CD11c^Hi CD8⁻ CD11b⁺ CD4⁻ (E) were derived from total cDCs. The frequencies of CD86⁺ and TGF-β⁺ were derived from their respective cDC subset. “X” labels time points where <2 sets of data were available. Data combined from 2 or 3 independent experiments (n = 5 per group) are shown. *P < .05, **P < .01, ***P < .001, ****P < .0001.