EEV counts during RBC unit (RBCu) storage at 4°C. (A) Mean PEV and EEV concentrations are compared between RBCu prepared in the Bourgogne Franche-Comté center and stored <7 days (red bars, n = 351), between 8 and 21 days (blue bars, n = 192), and >21 days (green bars, n = 105). (B) PEV and EEV concentrations according to days of storage. (C) EEV size and phenotype are compared in RBCu prepared at the Bourgogne Franche-Comté center and stored <7 days, between 8 and 21 days, and >21 days. Mean repartition of small (0.3-0.5 μm, pink bars) and large (0.5-0.9 μm, red bars), high (CD47^high, light blue bars) and low (CD47^low, blue bars) CD47 expression, and PS positive (annexin V–positive [A5⁺], light green bars) and negative (A5–, green bars) EEVs are presented. Percentages of small, CD47^high, and A5⁺ EEVs are noted. (D) Percentage of CD47^high expressing EEVs among total EEVs, according to storage duration for each RBCu. Pearson correlation is significant, P < .0001; r = 0.2545. (E) Percentage of small EEV among total EEVs according to storage duration for each RBCu. Pearson correlation is nonsignificant, P = .9182; r = 0.004. (F) Percentage of A5⁺ EEVs among total EEVs according to storage duration for each RBCu. Pearson correlation is nonsignificant (NS), P = .8928; r = –0.001. ***P < .0001 according to Student t test.