New ceramide analogs 315 and 403 induce intracellular ceramides production through regulation of ceramide synthases and enzymes of ceramide metabolism. (A-D) BCBL-1 (A-B) and BCP-1 (C-D) cells were treated with 20 µM of analog 315, 403, or vehicle for 24 hours. Ceramide species and total levels were then quantified using lipidomics analyses as described in the “Methods.” Error bars represent SD for 2 independent experiments. *P < .05; **P < .01. (E-F) Relative compositions and proportions of specific ceramide species were present within vehicle-, 315-, or 403-treated PEL cell lines. Each color representing a specific ceramide species is labeled beside the pie chart. (G) BCBL-1 and BCP-1 cells were treated with 20 µM of analog 315, 403, or vehicle for 24 hours; then the gene transcription was quantified using qRT-PCR. (H-I) The protein expression was detected using immunoblots. Error bars represent SD for 3 independent experiments. *P < .05; **P < .01.