Effects of hepcidin-mediated maternal iron restriction on embryo iron status. Embryos from PR73- and solvent-injected pregnancies from Figure 4 were analyzed at E18.5. (A) Serum iron. (B) Embryo liver non-heme iron. (C) Embryo brain nonheme iron concentration. (D) Embryo brain weight. (E) Total embryo brain nonheme iron content. (F) Embryo liver Saa1 mRNA expression. Statistical comparisons were performed by 2-tailed Student t test for normally distributed values, 2-tailed Mann-Whitney rank-sum test for nonnormally distributed values (indicated by * following P-values), or 2-tailed t test with Welch’s correction for normally distributed but nonequal variance data sets (indicated by ^# after the P value).