Effects of hepcidin-mediated maternal iron restriction on embryo outcome and hematological parameters. Embryos from PR73- and solvent-injected pregnancies from Figure 4 were analyzed at E18.5. (A) Embryos were visibly anemic, with increased mortality and resorption in the more severe 50-11d group. (B-E) Analysis of embryos from the 50-6d and 10-11d groups. (B) Embryo weight. (C-E) Blood hemoglobin concentration (C), RBC count (D), and mean corpuscular volume MCV (E). Statistical comparisons were performed by 2-tailed Student t test for normally distributed values or 2-tailed t test with Welch’s correction for normally distributed but non-equal variance data sets (indicated by ^# after the P value).