Systemic inflammation induces maternal hepcidin and severe hypoferremia during pregnancy. Age-matched nonpregnant and pregnant (E17.5) WT C57BL/6J females received a single subcutaneous injection of water or 0.5 µg/g LPS and were analyzed 6 hours later for maternal serum hepcidin (A) and maternal serum iron (B). Statistical comparisons were performed by 2-tailed Student t test for normally distributed values, 2-tailed Mann-Whitney rank-sum test for nonnormally distributed value (indicated by * following P-values), or 2-tailed t test with Welch’s correction for normally distributed but nonequal variance data sets (indicated by ^# after the P value).