Cecal ILC subsets express transcripts of Cβ, Cα, Cγ, and Cδ. (A) Activated ILC subsets were sort-purified based on Lin^–CD45.2⁺Thy1.2⁺CD3^– surface markers (green) for droplet-based scRNA-seq analyses. (B) Unsupervised clustering of ILC subsets using Seurat R package. t-distributed stochastic neighbor embedding (tSNE) plots were generated to visualize graph-based clustering of single cecum ILC libraries. NK, T, and B cells were excluded from the analysis based on their signature mRNA expression profiles. (C) Distribution of TCR constant region gene expression in activated ILC subsets.