Results of virologic investigations of the 3 SARS-CoV-2–positive blood donations
| No. of donation . | Detection by rtPCR on neat plasma, n positive/n tested (Ct) . | Detection by rtPCR after UC, n positive/n tested (Ct) . | RT-PCR‡/sequencing . | Virus culture . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| R-GENE PCR1* . | R-GENE PCR2* . | NRC rtPCR† . | R-GENE PCR1 . | R-GENE PCR2 . | ||||||||
| NC . | RdPd . | E . | RdPd target 1 . | RdPd target 2 . | NC . | RdPd . | E . | Plasma . | After UC . | Plasma . | After i-UC . | |
| 1 | POS 1/1 (37.9) | NEG 0/1 | NEG 0/1 | NEG 0/4 | Pos 2/4 (37.0; 37.1) | POS 1/1 (35.3) | POS 1/1 (41.6) | NEG O/1 | NEG | POS 1/1 | NEG | NEG |
| 2 | POS 1/1 (37.5) | NEG 0/1 | NEG 0/1 | POS 1/3 (39.3) | POS 1/3 (37.4) | POS 1/2 (37.0) | NEG 0/2 | NEG 0/1 | NEG | POS 1/2 | NEG | NEG |
| 3 | POS 1/1 (38.0) | NEG 0/1 | POS 1/1 (38.4) | NEG 0/2 | NEG 0/2 | POS 1/2 (38.3) | NEG 0/2 | POS 1/1 (42.2) | NEG | NEG 0/2 | NEG | NEG |
| No. of donation . | Detection by rtPCR on neat plasma, n positive/n tested (Ct) . | Detection by rtPCR after UC, n positive/n tested (Ct) . | RT-PCR‡/sequencing . | Virus culture . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| R-GENE PCR1* . | R-GENE PCR2* . | NRC rtPCR† . | R-GENE PCR1 . | R-GENE PCR2 . | ||||||||
| NC . | RdPd . | E . | RdPd target 1 . | RdPd target 2 . | NC . | RdPd . | E . | Plasma . | After UC . | Plasma . | After i-UC . | |
| 1 | POS 1/1 (37.9) | NEG 0/1 | NEG 0/1 | NEG 0/4 | Pos 2/4 (37.0; 37.1) | POS 1/1 (35.3) | POS 1/1 (41.6) | NEG O/1 | NEG | POS 1/1 | NEG | NEG |
| 2 | POS 1/1 (37.5) | NEG 0/1 | NEG 0/1 | POS 1/3 (39.3) | POS 1/3 (37.4) | POS 1/2 (37.0) | NEG 0/2 | NEG 0/1 | NEG | POS 1/2 | NEG | NEG |
| 3 | POS 1/1 (38.0) | NEG 0/1 | POS 1/1 (38.4) | NEG 0/2 | NEG 0/2 | POS 1/2 (38.3) | NEG 0/2 | POS 1/1 (42.2) | NEG | NEG 0/2 | NEG | NEG |
E, envelope protein; Ct, cycle threshold; i-UC, ultracentrifugation on iodixanol pad (10.5 mL plasma); NEG, negative; NC, nucleocapsid gene; POS, positive; RdPd, polymerase gene; UC, ultracentrifugation (12 mL plasma).
SARS-COV-2 R-GENE assay (BioMérieux).
rtPCR from the National Reference Center for respiratory viruses (NCR rt-PCR, Pasteur Institute).
RT-PCR primers: 5′-GCTTTTCAAACTGTCAAACC-3′ and 5′-GGTGAGGGTTTTCTACATCA-3′; hemi-nested PCR primers: 5′-TGCTGTGTCTAAGGGTTTCT-3′ and 5′-GGTGAGGGTTTTCTACATCA-3′.