Steady-state kinetics of fatty acids with 12-LOX
| Substrate . | kcat, s−1 . | KM, μM . | kcat/KM . | Relative kcat /KM* . |
|---|---|---|---|---|
| AA | 18 (0.8) | 0.95 (0.1) | 19 (2) | 1 |
| DHA | 13 (0.2) | 0.90 (0.06) | 14 (0.8) | 0.73 |
| DPAn-3 | 10 (0.3) | 2.3 (0.2) | 4.0 (2) | 0.22 |
| DPAn-6 | 13 (0.8) | 2.1 (0.3) | 6.2 (0.2) | 0.32 |
| Substrate . | kcat, s−1 . | KM, μM . | kcat/KM . | Relative kcat /KM* . |
|---|---|---|---|---|
| AA | 18 (0.8) | 0.95 (0.1) | 19 (2) | 1 |
| DHA | 13 (0.2) | 0.90 (0.06) | 14 (0.8) | 0.73 |
| DPAn-3 | 10 (0.3) | 2.3 (0.2) | 4.0 (2) | 0.22 |
| DPAn-6 | 13 (0.8) | 2.1 (0.3) | 6.2 (0.2) | 0.32 |
Steady-state kinetic parameters of AA, DHA, DPAn-3, and DPAn-6 with recombinant h12-LOX. Error is shown in parentheses.
Relative kcat/KM of h12-LOX with AA set to 1.