Table 2. Steady-state kinetics of fatty... | American Society of Hematology

Table 2.

Steady-state kinetics of fatty acids with 12-LOX

Substrate	k_cat, s⁻¹	K_M, μM	k_cat/K_M	Relative k_cat /K_M*
AA	18 (0.8)	0.95 (0.1)	19 (2)	1
DHA	13 (0.2)	0.90 (0.06)	14 (0.8)	0.73
DPA_n-3	10 (0.3)	2.3 (0.2)	4.0 (2)	0.22
DPA_n-6	13 (0.8)	2.1 (0.3)	6.2 (0.2)	0.32

Substrate	k_cat, s⁻¹	K_M, μM	k_cat/K_M	Relative k_cat /K_M*
AA	18 (0.8)	0.95 (0.1)	19 (2)	1
DHA	13 (0.2)	0.90 (0.06)	14 (0.8)	0.73
DPA_n-3	10 (0.3)	2.3 (0.2)	4.0 (2)	0.22
DPA_n-6	13 (0.8)	2.1 (0.3)	6.2 (0.2)	0.32

Steady-state kinetic parameters of AA, DHA, DPA_n-3, and DPA_n-6 with recombinant h12-LOX. Error is shown in parentheses.

Relative k_cat/K_M of h12-LOX with AA set to 1.