Hematologic parameters for WT mice and Tmprss6−/− mice that express full-length or mutated Mt2 by AAV8 vectors
| Parameter . | WT (n = 17) . | Tmprss6−/− + PBS (n = 26) . | Tmprss6−/−- + Mt2 (+) (n = 13) . | Tmprss6−/− + Mt2 (++) (n = 13) . | Tmprss6−/− + Mt2I286F (+) (n = 12) . | Tmprss6−/− + Mt2I286F (++) (n = 10) . | Tmprss6−/− + Mt2S762A (+) (n = 12) . | Tmprss6−/− + Mt2S762A (++) (n = 10) . |
|---|---|---|---|---|---|---|---|---|
| RBC, ×106/µL | 8.75 ± 0.41 | 10.65 ± 0.55*** | 10.82 ± 0.56*** | 10.38 ± 0.45*** | 10.86 ± 0.39*** | 10.78 ± 0.51*** | 10.55 ± 0.54*** | 10.16 ± 0.59*** |
| Hb, g/dL | 13.09 ± 0.51 | 8.42 ± 0.76*** | 11.88 ± 0.82** | 12.56 ± 0.80 | 9.74 ± 0.37*** | 12.01 ± 0.65** | 11.63 ± 0.64** | 12.55 ± 0.53 |
| HCT, % | 48.69 ± 2.72 | 36.76 ± 2.13*** | 48.55 ± 3.66 | 52.27 ± 3.89 | 39.87 ± 2.59*** | 49.35 ± 3.79 | 45.63 ± 3.69 | 50.42 ± 1.97 |
| MCV, fL | 55.21 ± 2.87 | 34.52 ± 2.45*** | 45.58 ± 2.79*** | 50.89 ± 4.45* | 36.91 ± 2.27*** | 46.47 ± 2.88*** | 43.4 ± 2.97*** | 51.04 ± 3.41* |
| MCH, pg | 14.98 ± 0.57 | 7.93 ± 0.85*** | 11.19 ± 1.08*** | 12.45 ± 1.17*** | 8.75 ± 0.86*** | 11.11 ± 0.86*** | 11.09 ± 0.88*** | 12.56 ± 0.96*** |
| Parameter . | WT (n = 17) . | Tmprss6−/− + PBS (n = 26) . | Tmprss6−/−- + Mt2 (+) (n = 13) . | Tmprss6−/− + Mt2 (++) (n = 13) . | Tmprss6−/− + Mt2I286F (+) (n = 12) . | Tmprss6−/− + Mt2I286F (++) (n = 10) . | Tmprss6−/− + Mt2S762A (+) (n = 12) . | Tmprss6−/− + Mt2S762A (++) (n = 10) . |
|---|---|---|---|---|---|---|---|---|
| RBC, ×106/µL | 8.75 ± 0.41 | 10.65 ± 0.55*** | 10.82 ± 0.56*** | 10.38 ± 0.45*** | 10.86 ± 0.39*** | 10.78 ± 0.51*** | 10.55 ± 0.54*** | 10.16 ± 0.59*** |
| Hb, g/dL | 13.09 ± 0.51 | 8.42 ± 0.76*** | 11.88 ± 0.82** | 12.56 ± 0.80 | 9.74 ± 0.37*** | 12.01 ± 0.65** | 11.63 ± 0.64** | 12.55 ± 0.53 |
| HCT, % | 48.69 ± 2.72 | 36.76 ± 2.13*** | 48.55 ± 3.66 | 52.27 ± 3.89 | 39.87 ± 2.59*** | 49.35 ± 3.79 | 45.63 ± 3.69 | 50.42 ± 1.97 |
| MCV, fL | 55.21 ± 2.87 | 34.52 ± 2.45*** | 45.58 ± 2.79*** | 50.89 ± 4.45* | 36.91 ± 2.27*** | 46.47 ± 2.88*** | 43.4 ± 2.97*** | 51.04 ± 3.41* |
| MCH, pg | 14.98 ± 0.57 | 7.93 ± 0.85*** | 11.19 ± 1.08*** | 12.45 ± 1.17*** | 8.75 ± 0.86*** | 11.11 ± 0.86*** | 11.09 ± 0.88*** | 12.56 ± 0.96*** |
Data are presented as the mean ± standard deviation. One-way analysis of variance was used to analyze the data relative to wild-type (WT) mice.
Hb, hemoglobin; HCT, hematocrit; MCH, mean corpuscular hemoglobin; MCV, mean cell volume; RBC, red blood cells.
*P < .05; **P < .01; ***P < .001.