Mouse ORF or mutant constructs used for transfection and generation of AAV8 vectors, as well as the antibodies used for western blot analysis
| Mouse constructs . | Encoded proteins . | Tag epitope . | Antibodies used for western blot analysis . |
|---|---|---|---|
| ActRIIA | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Alk2 | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Alk3 | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Bmpr2 | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Hai-2 | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Hfe | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Hjv | GPI-anchored membrane protein | N-terminal 3xFLAG | Anti-FLAG and anti-HJV |
| Mt2 | Type II transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Mt2I286F | Type II transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Mt2mask | Type II transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Mt2S762A | Type II transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Tfr2 | Type II transmembrane protein | No tag | Anti-Tfr2 |
| Zip14 | Membrane protein with 7 transmembrane segments | C-terminal 3xFLAG | Anti-FLAG |
| Mouse constructs . | Encoded proteins . | Tag epitope . | Antibodies used for western blot analysis . |
|---|---|---|---|
| ActRIIA | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Alk2 | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Alk3 | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Bmpr2 | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Hai-2 | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Hfe | Type I transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Hjv | GPI-anchored membrane protein | N-terminal 3xFLAG | Anti-FLAG and anti-HJV |
| Mt2 | Type II transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Mt2I286F | Type II transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Mt2mask | Type II transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Mt2S762A | Type II transmembrane protein | C-terminal FLAG/MYC | Anti-FLAG |
| Tfr2 | Type II transmembrane protein | No tag | Anti-Tfr2 |
| Zip14 | Membrane protein with 7 transmembrane segments | C-terminal 3xFLAG | Anti-FLAG |