Table 1.

HLA-A*02:01-restricted TAAs and the MiHA included in this study

ProteinPeptidePeptide binding affinity for HLA-A*02:01, nM
TAA   
 WT1 RMFPNAPYL 7.14 (SB) 
 RHAMM ILSLELMKL 160.52 (WB) 
 Proteinase-3 VLQELNVTV 12.33 (SB) 
 PRAME VLDGLDVLL 51.09 (WB) 
 NY-eso-1 SLLMWITQV 6.13 (WB) 
MiHA   
 HA-1 VLHDDLLEA 28.61 (SB) 
ProteinPeptidePeptide binding affinity for HLA-A*02:01, nM
TAA   
 WT1 RMFPNAPYL 7.14 (SB) 
 RHAMM ILSLELMKL 160.52 (WB) 
 Proteinase-3 VLQELNVTV 12.33 (SB) 
 PRAME VLDGLDVLL 51.09 (WB) 
 NY-eso-1 SLLMWITQV 6.13 (WB) 
MiHA   
 HA-1 VLHDDLLEA 28.61 (SB) 

MHC I-Streptamers for isolation and tetramers for flow cytometry analysis were available for the indicated peptides. Peptide-binding affinities for HLA-A*02:01 were predicted by using NetMHC Version 4.0. SB are predicted strong binders, and WB are predicted weak binders.

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