Iron parameters of Tfr2BMKO/Hbbth3/+mice 9 and 22 weeks after BMT
| . | . | TS, % . | LIC, μg/g . | SIC, μg/g . | KIC, μg/g . | HIC, μg/g . | Hamp −ΔCt . | Hamp/LIC . |
|---|---|---|---|---|---|---|---|---|
| 9 wk after BMT | Hbbth3/+ | 49.9 ± 4.5 | 529.2 ± 23.7 | 4039 ± 69 | 248.0 ± 29.3 | 229.2 ± 10.8 | 5.79 ± 0.12 | 1.01 ± 0.09 |
| Tfr2BMKO/Hbbth3/+ | 41.1 ± 2.3 | 530.8 ± 72.4 | 3981 ± 172 | 249.8 ± 23.8 | 215.7 ± 15.1 | 5.90 ± 0.24 | 1.18 ± 0.14 | |
| 22 wk after BMT | Hbbth3/+ | 31.1 ± 1.7 | 961.3 ± 98.2 | 7234 ± 779 | 401.3 ± 38.1 | 388.7 ± 19.4 | 5.03 ± 0.29 | 0.50 ± 0.05 |
| Tfr2BMKO/Hbbth3/+ | 30.3 ± 5.3 | 658.6 ± 83.0* | 2383 ± 647*** | 263.7 ± 21.4* | 382.9 ± 29.7 | 2.80 ± 0.96* | 0.31 ± 0.19 |
| . | . | TS, % . | LIC, μg/g . | SIC, μg/g . | KIC, μg/g . | HIC, μg/g . | Hamp −ΔCt . | Hamp/LIC . |
|---|---|---|---|---|---|---|---|---|
| 9 wk after BMT | Hbbth3/+ | 49.9 ± 4.5 | 529.2 ± 23.7 | 4039 ± 69 | 248.0 ± 29.3 | 229.2 ± 10.8 | 5.79 ± 0.12 | 1.01 ± 0.09 |
| Tfr2BMKO/Hbbth3/+ | 41.1 ± 2.3 | 530.8 ± 72.4 | 3981 ± 172 | 249.8 ± 23.8 | 215.7 ± 15.1 | 5.90 ± 0.24 | 1.18 ± 0.14 | |
| 22 wk after BMT | Hbbth3/+ | 31.1 ± 1.7 | 961.3 ± 98.2 | 7234 ± 779 | 401.3 ± 38.1 | 388.7 ± 19.4 | 5.03 ± 0.29 | 0.50 ± 0.05 |
| Tfr2BMKO/Hbbth3/+ | 30.3 ± 5.3 | 658.6 ± 83.0* | 2383 ± 647*** | 263.7 ± 21.4* | 382.9 ± 29.7 | 2.80 ± 0.96* | 0.31 ± 0.19 |
Mice were analyzed 9 and 22 weeks after transplantation with thalassemic (Hbbth3/+) or Tfr2−/−/Hbbth3/+ (Tfr2BMKO/Hbbth3/+) BM. Mice were fed a standard diet. The table reports: TS, LIC, SIC, KIC, HIC, and mRNA expression of hepcidin (Hamp) relative to Hprt1 and relative to LIC (Hamp/LIC). Mean values plus or minus SEs are indicated.
HIC, heart iron content; Hprt1, hypoxanthine phosphoribosyltransferase 1; KIC, kidney iron content; mRNA, messenger RNA.
Asterisks refer to statistically significant differences between age-matched Tfr2BMKO/Hbbth3/+ and Hbbth3/+ mice: *P < .05; ***P < .005.