Table 2. Sequence, exons covered,... | American Society of Hematology

Table 2.

Sequence, exons covered, product sizes, and annealing temperature for primers used for RHAG LR-PCR

Primer name	Sequence 5′-3′	Exons	Size, bp	Annealing temperature, °C
RHAG-1 forward	TGGTAGGGCTGATTTCCTTGT	6, 7, 8, 9, 10	10 003	62
RHAG-1 reverse	TGGATGTTTTGGCCCAGCTT	6, 7, 8, 9, 10	10 003	62
RHAG-2 forward	GCTGATCTGAGGGTTACTCCTTT	2, 3, 4, 5	10 519	62
RHAG-2 reverse	AGGAGGATGGGAACGCTAAG	2, 3, 4, 5	10 519	62
RHAG-3 forward	AATTATTCTGCAGATTTCACCCC	1	15 083	62
RHAG-3 reverse	GGAGACAAGAATTCCTCCACCTAT	1	15 083	62

Primer name	Sequence 5′-3′	Exons	Size, bp	Annealing temperature, °C
RHAG-1 forward	TGGTAGGGCTGATTTCCTTGT	6, 7, 8, 9, 10	10 003	62
RHAG-1 reverse	TGGATGTTTTGGCCCAGCTT	6, 7, 8, 9, 10	10 003	62
RHAG-2 forward	GCTGATCTGAGGGTTACTCCTTT	2, 3, 4, 5	10 519	62
RHAG-2 reverse	AGGAGGATGGGAACGCTAAG	2, 3, 4, 5	10 519	62
RHAG-3 forward	AATTATTCTGCAGATTTCACCCC	1	15 083	62
RHAG-3 reverse	GGAGACAAGAATTCCTCCACCTAT	1	15 083	62