Expression models/strains used in this study
| Model/strain name . | Description . | Deletions . | Lentiviral expression . | Transgenic expression . | Genetic background . |
|---|---|---|---|---|---|
| WT | Wild type | C57BL/6 | |||
| FVIIInull | Total mF8 deletion | mF8 | C57BL/6 | ||
| 2bF8LV | Total mF8 deletion, platelet-specific LV hF8 expression | mF8 | 2bF8LV via the αIIb promotor | B6/129S | |
| T2F8LV | Total mF8 deletion, endothelial-specific LV hF8 expression | mF8 | T2F8LV via the Tie-2 promotor | B6/129S | |
| 2bF8tg+/− | Total mF8 deletion, platelet-specific Tg hF8 expression | mF8 | hF8 via αIIb promotor | B6/129S | |
| T2F8tg+/− (line: ES#5) | Total mF8 deletion, EC-specific Tg hF8 expression (1 copy) | mF8 | hF8 via Tie-2 promotor | B6/129S | |
| T2F8tg+/+ (line: ES#5) | Total mF8 deletion, EC-specific Tg hF8 expression (2 copies) | mF8 | hF8 via Tie-2 promotor | B6/129S | |
| T2F8tg+/+ (line: ES #65) | Total mF8 deletion, EC-specific Tg hF8 expression (5 copies) | mF8 | hF8 via Tie-2 promotor | B6/129S | |
| T2F8TgF8−/−VWF−/− | Total mF8 and VWF deletions, EC-specific Tg hF8 expression | mF8, mVWF | hF8 via Tie-2 promotor | B6/129S | |
| CIITA−/− | Total CIITA deletion (MHCII null) | CIITA (MHCII transactivator) | C57BL/6 |
| Model/strain name . | Description . | Deletions . | Lentiviral expression . | Transgenic expression . | Genetic background . |
|---|---|---|---|---|---|
| WT | Wild type | C57BL/6 | |||
| FVIIInull | Total mF8 deletion | mF8 | C57BL/6 | ||
| 2bF8LV | Total mF8 deletion, platelet-specific LV hF8 expression | mF8 | 2bF8LV via the αIIb promotor | B6/129S | |
| T2F8LV | Total mF8 deletion, endothelial-specific LV hF8 expression | mF8 | T2F8LV via the Tie-2 promotor | B6/129S | |
| 2bF8tg+/− | Total mF8 deletion, platelet-specific Tg hF8 expression | mF8 | hF8 via αIIb promotor | B6/129S | |
| T2F8tg+/− (line: ES#5) | Total mF8 deletion, EC-specific Tg hF8 expression (1 copy) | mF8 | hF8 via Tie-2 promotor | B6/129S | |
| T2F8tg+/+ (line: ES#5) | Total mF8 deletion, EC-specific Tg hF8 expression (2 copies) | mF8 | hF8 via Tie-2 promotor | B6/129S | |
| T2F8tg+/+ (line: ES #65) | Total mF8 deletion, EC-specific Tg hF8 expression (5 copies) | mF8 | hF8 via Tie-2 promotor | B6/129S | |
| T2F8TgF8−/−VWF−/− | Total mF8 and VWF deletions, EC-specific Tg hF8 expression | mF8, mVWF | hF8 via Tie-2 promotor | B6/129S | |
| CIITA−/− | Total CIITA deletion (MHCII null) | CIITA (MHCII transactivator) | C57BL/6 |
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