Effects of 17R-RvD1 on lung and kidney pathology of AA and SS mice under normoxic conditions and exposure to H/R stress
| . | AA mice . | SS mice . | ||||
|---|---|---|---|---|---|---|
| . | Normoxia . | H/R . | H/R Rvs . | Normoxia . | H/R . | H/R Rvs . |
| Lung | (n = 3) | (n = 6) | (n = 3) | (n = 3) | (n = 5) | (n = 3) |
| Inflammatory cell infiltrates, mean ± SD | 6.4 ± 1.9 | 25.2 ± 3.0* | 4.9 ± 1.7† | 9.0 ± 1.2‡ | 78.2 ± 3.5*,‡ | 36.1 ± 8.0*,†,‡ |
| Edema | 0 | 0 | 0 | 0 | 0 | 0 |
| Mucus | 0 | + (3/6) | 0 | 0 | + (1/5) | 0 |
| Thrombi | 0 | 0 | 0 | 0 | + (4/5) | + (3/3) |
| Kidney | (n = 3) | (n = 6) | (n = 3) | (n = 3) | (n = 5) | (n = 3) |
| Inflammatory cell infiltrates, mean ± SD | 0 | 0.4 ± 0.03* | 0† | 0 | 2.9 ± 0.02*,‡ | 1.7 ± 0.06†,‡ |
| Thrombi | 0 | 0 | 0 | 0 | + (2/5) | 0 |
| Sclerotic glomerula | 0 | + (1/6) | 0 | 0 | + (3/5) | 0 |
| Necrosis | 0 | 0 | 0 | 0 | + (2/5) | 0 |
| . | AA mice . | SS mice . | ||||
|---|---|---|---|---|---|---|
| . | Normoxia . | H/R . | H/R Rvs . | Normoxia . | H/R . | H/R Rvs . |
| Lung | (n = 3) | (n = 6) | (n = 3) | (n = 3) | (n = 5) | (n = 3) |
| Inflammatory cell infiltrates, mean ± SD | 6.4 ± 1.9 | 25.2 ± 3.0* | 4.9 ± 1.7† | 9.0 ± 1.2‡ | 78.2 ± 3.5*,‡ | 36.1 ± 8.0*,†,‡ |
| Edema | 0 | 0 | 0 | 0 | 0 | 0 |
| Mucus | 0 | + (3/6) | 0 | 0 | + (1/5) | 0 |
| Thrombi | 0 | 0 | 0 | 0 | + (4/5) | + (3/3) |
| Kidney | (n = 3) | (n = 6) | (n = 3) | (n = 3) | (n = 5) | (n = 3) |
| Inflammatory cell infiltrates, mean ± SD | 0 | 0.4 ± 0.03* | 0† | 0 | 2.9 ± 0.02*,‡ | 1.7 ± 0.06†,‡ |
| Thrombi | 0 | 0 | 0 | 0 | + (2/5) | 0 |
| Sclerotic glomerula | 0 | + (1/6) | 0 | 0 | + (3/5) | 0 |
| Necrosis | 0 | 0 | 0 | 0 | + (2/5) | 0 |
Inflammatory cell infiltrates in the lung are expressed as the mean number of cells per field of view at original magnification ×250, resulting from the analysis of ≥4 fields of view from each hematoxylin and eosin–stained whole-lung section (see also de Franceschi et al28,29 ). Edema: 0, no edema. Mucus: 0, no mucus; +, mucus filling <25% of the area of the bronchus circumference at original magnification ×400. Thrombi: 0, no thrombi; +, presence of thrombus per field at original magnification ×250. Quantification of inflammatory cell infiltration in the renal cortex of kidney was determined in hematoxylin and eosin–stained sections using a scale (0-4) based on the percentage of each field occupied by cell infiltrates: 0 (no sign of infiltration), 1 (1-10%), 2 (11-25%), 3 (26-50%), and 4 (>50%). The mean of 15 randomly selected fields was analyzed at original magnification ×400 (see also Sabaa et al22 and Guo et al46 ). Sclerotic glomerula: 0, no sclerotic glomerula; +, >50% sclerotic glomerula. Necrosis: 0, no necrosis; +, presence of necrosis.
SD, standard deviation.
P < .05 vs normoxia, †P < .05 vs vehicle, ‡P < .05 vs healthy mice (AA), 2-way ANOVA algorithm for repeated measures combined with the Bonferroni correction.