N-gly site status in most and least diverse subclone based on degree of SHM compared with V gene germline sequence
| . | Patient 1 . | Patient 2 . | Patient 3 . | Patient 4 . | Patient 5 . | Patient 6 . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Least mutated subclone | FL | tFL | FL1 | FL3 | tFL | FL2 | FL3 | tFL | FL1 | FL2 | FL1 | FL3 | FL | tFL | tFL1 |
| Percentage homology to G.L V gene sequence | 96.4 | 98.0 | 88.7 | 88.3 | 94.4 | 84.7 | 86.1 | 95.6 | 89.4 | 89.0 | 98.0 | 89.1 | 97.8 | 93.0 | 92.3 |
| N-gly site presence (Y/N) | Y | Y | Y, Y, Y, Y | Y, Y, Y, N | N, N, N, Y | Y | Y | N | Y,Y | Y, Y | N | Y (FL1 site) | Y | Y | Y |
| Most mutated subclone | |||||||||||||||
| Percentage homology to G.L V gene sequence | 84.3 | 83.9 | 84.7 | 84.3 | 84.3 | 79.0 | 79.4 | 79.6 | 87.4 | 86.9 | 87.5 | 81.5 | 79.4 | 71.3 | 73.5 |
| N-gly site presence (Y/N) | Y | Y | Y, Y, Y, Y | Y, Y, Y, Y | Y, Y, Y, Y | Y | Y | Y | Y,Y | Y,Y | Y | Y | Y | Y | Y |
| Percentage difference in homology between least and most mutated subclones | 12.1 | 14.1 | 4.0 | 4.0 | 10.1 | 5.7 | 6.7 | 16.0 | 2.0 | 2.1 | 10.5 | 7.6 | 18.4 | 21.7 | 18.8 |
| . | Patient 1 . | Patient 2 . | Patient 3 . | Patient 4 . | Patient 5 . | Patient 6 . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Least mutated subclone | FL | tFL | FL1 | FL3 | tFL | FL2 | FL3 | tFL | FL1 | FL2 | FL1 | FL3 | FL | tFL | tFL1 |
| Percentage homology to G.L V gene sequence | 96.4 | 98.0 | 88.7 | 88.3 | 94.4 | 84.7 | 86.1 | 95.6 | 89.4 | 89.0 | 98.0 | 89.1 | 97.8 | 93.0 | 92.3 |
| N-gly site presence (Y/N) | Y | Y | Y, Y, Y, Y | Y, Y, Y, N | N, N, N, Y | Y | Y | N | Y,Y | Y, Y | N | Y (FL1 site) | Y | Y | Y |
| Most mutated subclone | |||||||||||||||
| Percentage homology to G.L V gene sequence | 84.3 | 83.9 | 84.7 | 84.3 | 84.3 | 79.0 | 79.4 | 79.6 | 87.4 | 86.9 | 87.5 | 81.5 | 79.4 | 71.3 | 73.5 |
| N-gly site presence (Y/N) | Y | Y | Y, Y, Y, Y | Y, Y, Y, Y | Y, Y, Y, Y | Y | Y | Y | Y,Y | Y,Y | Y | Y | Y | Y | Y |
| Percentage difference in homology between least and most mutated subclones | 12.1 | 14.1 | 4.0 | 4.0 | 10.1 | 5.7 | 6.7 | 16.0 | 2.0 | 2.1 | 10.5 | 7.6 | 18.4 | 21.7 | 18.8 |
Sequences were analyzed for presence or absence of the N-gly motif site or sites found in the major clone.