Summary of VDJ sequencing results for patients 1 to 6
| Patient . | Disease event . | Dominant VDJ rearrangement . | No. of unique VDJ rearrangements . | Dominant rearrangement (% of total reads) . | No. of unique subclones with dominant VDJ rearrangement . |
|---|---|---|---|---|---|
| 1 | Diagnosis | V3-30, D3-16, J6 | 29 | 99.03 | 1690 |
| Transformation | V3-30, D3-16, J6 | 313 | 94.95 | 2727 | |
| 2 | First relapse | V3-11, D3-16, J1 | 105 | 95.04 | 3530 |
| Third relapse | V3-11, D3-16, J1 | 12 | 99.33 | 3032 | |
| Transformation | V3-11, D3-16, J1 | 129 | 99.23 | 3966 | |
| 3 | Second relapse | V3-48, D1-26, J4 | 164 | 88.23 | 4103 |
| Third relapse | V3-48, D1-26, J4 | 261 | 71.10 | 2057 | |
| Transformation | V3-48, D1-26, J4 | 143 | 84.81 | 1592 | |
| 4 | First relapse | V3-48, D3-10, J6 | 101 | 56.60 | 81 |
| Second relapse | V3-48, D3-10, J6 | 324 | 69.70 | 248 | |
| 5 | First relapse | V3-23, D4-23, J6 | 224 | 53.30 | 140 |
| Third relapse | V3-23, D3-3*, J5* | 433 | 70.70 | 371 | |
| 6 | FL diagnosis | V3-23, D5-18, J6 | 7,931 | 82.40 | 2510 |
| tFL diagnosis | V3-23, D5-18, J6 | 2,436 | 89.60 | 3442 | |
| tFL relapse | V3-23, D5-18, J6 | 1,204 | 93.10 | 4749 |
| Patient . | Disease event . | Dominant VDJ rearrangement . | No. of unique VDJ rearrangements . | Dominant rearrangement (% of total reads) . | No. of unique subclones with dominant VDJ rearrangement . |
|---|---|---|---|---|---|
| 1 | Diagnosis | V3-30, D3-16, J6 | 29 | 99.03 | 1690 |
| Transformation | V3-30, D3-16, J6 | 313 | 94.95 | 2727 | |
| 2 | First relapse | V3-11, D3-16, J1 | 105 | 95.04 | 3530 |
| Third relapse | V3-11, D3-16, J1 | 12 | 99.33 | 3032 | |
| Transformation | V3-11, D3-16, J1 | 129 | 99.23 | 3966 | |
| 3 | Second relapse | V3-48, D1-26, J4 | 164 | 88.23 | 4103 |
| Third relapse | V3-48, D1-26, J4 | 261 | 71.10 | 2057 | |
| Transformation | V3-48, D1-26, J4 | 143 | 84.81 | 1592 | |
| 4 | First relapse | V3-48, D3-10, J6 | 101 | 56.60 | 81 |
| Second relapse | V3-48, D3-10, J6 | 324 | 69.70 | 248 | |
| 5 | First relapse | V3-23, D4-23, J6 | 224 | 53.30 | 140 |
| Third relapse | V3-23, D3-3*, J5* | 433 | 70.70 | 371 | |
| 6 | FL diagnosis | V3-23, D5-18, J6 | 7,931 | 82.40 | 2510 |
| tFL diagnosis | V3-23, D5-18, J6 | 2,436 | 89.60 | 3442 | |
| tFL relapse | V3-23, D5-18, J6 | 1,204 | 93.10 | 4749 |
Although samples of patient 5 have different DJ rearrangements according to IMGT, when aligned they show highly similar CDR3 regions and share a t(14:18) breakpoint, indicating a clonal relationship.
For patients 4 and 5, information in table was obtained from Carlotti et al,16 and patient 6 information was obtained from Jiang et al.17