Table 2.

Sequence library of the reciprocal (DH/mbr) junctions

Clone*DH S†DH sequence De novo nucleotide additions‡BCL-2 mbr sequence2-153mbr2-155S
Germline D1-7   GGTATAACTGGAACTAC  ACGTGGCCTGTTTCAACACAGACCCACCCAGAGC  1a  
Germline  D1-26  GGTATAGTGGGAGCTACTAC CCTCCTGCCCTCCTTCCGCGGGG 1b  
Germline D2-2   AGGATATTGTAGTAGTACCAGCTGCTATACC GCTTTCTCATGGCTGTCCTTCAGGGTCTTCCTGAAATG  2a  
Germline D2-8   AGGATATTGTACTAATGGTGTATGCTATACC   CAGTGGTCGTTACGCTCC 2b  
Germline  D2-15  AGGATATTGTAGTGGTGGTAGCTGCTACTCC ACCAAGAAAGCAGGAAACCTGTGGTATGAAGC  3a  
Germline D2-21   AGCATATTGTGGTGGTGACTGCTATTCC CAGACCTCCCCGGCGGGCCTCAGGGAACAGAATGATCAGAC  3b 
Germline  D3-3   GTATTACGATTTTTGGAGTGGTTATTATACC 
Germline  D3-9   GTATTACGATATTTTGACTGGTTATTATAAC 
Germline  D3-10   GTATTACTATGGTTCGGGGAGTTATTATAAC 
Germline  D3-16  GTATTATGATTACGTTTGGGGGAGTTATCGTTATACC  
Germline D3-22   GTATTACTATGATAGTAGTGGTTATTACTAC  
Germline D4-4   TGACTACAGTAACTAC  
Germline  D4-17  TGACTACGGTGACTAC  
Germline  D5-5/18  GTGGATACAGCTATGGTTAC  
Germline  D7-27  CTAACTGGGGA  
#1  D3-3  −4 GTATTACGATTTTTGGAGTGGTTATTA  ACT TCCTGCCCTCCTTCCGCGGGG 1b  −11  
#2*+ D3-22  0  TTACTATGATAGTAGTGGTTATTACTAC  TGTGGGTTGA GCAGGG 1b  −8  
#3*+  D2-2  −6 AGGATATTGTAGTAGTACCAGCTGC  CCACCGT GCGGGG 1b  −7  
#4+  D5-5/18  −5 GTGGATACAGCTATG  AGGTGAAAAACCACCCCCCAAG AACACAGACCCACCCAGAGC  1a  +34  
#5  D2-2  −9 AGGATATTGTAGTAGTACCAGC  CCAGCCTTC CGGGG 1b  −2  
#6*+  D3-3  −8 GTATTACGATTTTTGGAGTGGTT  TAACCAACTCT CGCGGGG 1b  +1  
#7* D7-27  0 CTAACTGGGGA  CACCCTTACCTATA CCGCGGGG 1b  +2  
#8+  D3-9  −6  GTATTACGATATTTTGACTGGTTAT AAGATCCAGG GGGG 1b  −2  
#9*+ D2-2  −5  AGGATATTGTAGTAGTACCAGCTGCT TCCGACCCCTGTGATCTAC CCGCGGGG 1b +4  
#10*+  D5-5/18  −2  GTGGATACAGCTATGGTT CCTTTTCGGTGGCCAACCGACAG GGGG 1b 0  
#11  D3-10  −12  GTATTACTATGGTTCGGGG   GGGG 1b  0  
#12  D3-3  −3 GTATTACGATTTTTGGAGTGGTTATTAT  TGTTCGGCCAAATAC GGG 1b  +1  
#13+  D3-9  −7 GTATTACGATATTTTGACTGGTTA  GCAATTCGGGGATTGGTAATGAGAAA GCGGGG 1b  +4  
#14  D3-3  −7 GTATTACGATTTTTGGAGTGGTTA CCTGGCGCCGCTATTCGGTAGGCGGACCCAAAAAGATAAGGGCCCCGACGAGTTTGCATGA GCTGTCCTTCAGGGTCTTCCTGAAATG  2a  −9  
#15  D4-4 −7  TGACTACAG   GTCTTCCTGAAATG  2a  +8  
#16 D5-5/18  −1  GTGGATACAGCTATGGTTA  TTGGCCT GCTCC 2b  −11  
#17* D2-8  −3 AGGATATTGTACTAATGGTGTATGCTAT  GTAGGGG TCC 2b  −5  
#18  D2-15  −1 ATTGTAGTGGTGGTAGCTGCTACTC  GTGTACTACTCTTGGGGGGGC GAAATG  2a  +17  
#19  D2-2  −12 AGGATATTGTAGTAGTACC  GGGTCGGTAGGG AAGCAGGAAACCTGTGGTATGAAGC  3a  −14  
#20  D4-17 −1  TGACTACGGTGACTA  GCCTCCCTGCCATACCAACGCCCTTC CGCTCC 2b  0  
#21  D2-15  −19 AGGATATTGTAG  CCCAAGCATAGGGTG CCAAGAAAGCAGGAAACCTGTGGTAT  3a  −7  
#22*+  D2-21 −10  AGCATATTGTGGTGGTGA  TTTGAAGCAGGGGGGCTT CTCC 2b  −2  
#23+  D3-3  −4 GTATTACGATTTTTGGAGTGGTTATTA CGGGTCGGTCCGAACCGCAACAGGGGGTTCTTTGTC CC 2b  −4  
#24  D2-2  −11  AGGATATTGTAGTAGTACCA CCG CGCTCC 2b  0  
#25+ D1-26  −5  GGTATAGTGGGAGCT  CTACAAGGGGACTCTCCC CTCC 2b  −2  
#26+  D4-4  0 TGACTACAGTAACTAC  TTTC CGCTCC 2b 0  
#27+  D2-2  −4  AGGATATTGTAGTAGTACCAGCTGCTA CCCAACCCCATG ACGCTCC 2b  +1 
#28  D3-3  −32  cactgt TTCGGAAGTTGTGCCAACGACCACA GCTCC 2b 0  
#29  D3-10  −6  GTATTACTATGGTTCGGGGAGTTAT CGTGTGGGTTTTGGGAGACGCAGTCCC  CC 2b −3  
#30+  D1-7  0  GGTATAACTGGAACTAC CTCACCTTCC TCC 2b  −2  
#31+ D3-9  −6  GTATTACGATATTTTGACTGGTTAT  AACCTCTTAAG GTGGTATGAAGC  3a  −10  
#32  D2-2  −3 AGGATATTGTAGTAGTACCAGCTGCTAT  TTGTCTTTGTTACCTCTTTCATTTT TGTGGTATGAAGC  3a  −6  
#33*+  D2-2  −8 AGGATATTGTAGTAGTACCAGCT  TCGGTCC ACCTGTGGTATGAAGC  3a  −2  
#34  D2-2  −8  AGGATATTGTAGTAGTACCAGCT  CC 2b  +18  
#35*+  D3-3  −6 GTATTACGATTTTTGGAGTGGTTAT  CAGGGTAG GGGCCTCAGGGAACAGAATGATCAGAC  3b  −12  
#36  D3-3 −3  GTATTACGATTTTTGGAGTGGTTATTAT  TTCGATCACGCCGG TGATCAGAC  3b  −22  
#37  D3-16  −3 TTACGTTTGGGGGAGTTATCGTTAT  GTGGTCCCTTCCCGCTATA GGCGGGCCTCAGGGAACAGAATGATCAG  3b  0  
#38*+ JH6sp   ggtttttgtggggtgaggatggaca CCCTACAACGCGCGCACAAGACGT GCGGGCCTCAGGGAACAGAATGATCAG  3b  +4  
#39 D3-9  −7  GTATTACGATATTTTGACTGGTTA  CGGGCTTCC GAATGATCAGAC  3b  −13  
#40  D3-10  −6 GTATTACTATGGTTCGGGGAGTTAT  GTTTG GCAGGAAACCTGTGGTATGAAGC  3a  +56 
Clone*DH S†DH sequence De novo nucleotide additions‡BCL-2 mbr sequence2-153mbr2-155S
Germline D1-7   GGTATAACTGGAACTAC  ACGTGGCCTGTTTCAACACAGACCCACCCAGAGC  1a  
Germline  D1-26  GGTATAGTGGGAGCTACTAC CCTCCTGCCCTCCTTCCGCGGGG 1b  
Germline D2-2   AGGATATTGTAGTAGTACCAGCTGCTATACC GCTTTCTCATGGCTGTCCTTCAGGGTCTTCCTGAAATG  2a  
Germline D2-8   AGGATATTGTACTAATGGTGTATGCTATACC   CAGTGGTCGTTACGCTCC 2b  
Germline  D2-15  AGGATATTGTAGTGGTGGTAGCTGCTACTCC ACCAAGAAAGCAGGAAACCTGTGGTATGAAGC  3a  
Germline D2-21   AGCATATTGTGGTGGTGACTGCTATTCC CAGACCTCCCCGGCGGGCCTCAGGGAACAGAATGATCAGAC  3b 
Germline  D3-3   GTATTACGATTTTTGGAGTGGTTATTATACC 
Germline  D3-9   GTATTACGATATTTTGACTGGTTATTATAAC 
Germline  D3-10   GTATTACTATGGTTCGGGGAGTTATTATAAC 
Germline  D3-16  GTATTATGATTACGTTTGGGGGAGTTATCGTTATACC  
Germline D3-22   GTATTACTATGATAGTAGTGGTTATTACTAC  
Germline D4-4   TGACTACAGTAACTAC  
Germline  D4-17  TGACTACGGTGACTAC  
Germline  D5-5/18  GTGGATACAGCTATGGTTAC  
Germline  D7-27  CTAACTGGGGA  
#1  D3-3  −4 GTATTACGATTTTTGGAGTGGTTATTA  ACT TCCTGCCCTCCTTCCGCGGGG 1b  −11  
#2*+ D3-22  0  TTACTATGATAGTAGTGGTTATTACTAC  TGTGGGTTGA GCAGGG 1b  −8  
#3*+  D2-2  −6 AGGATATTGTAGTAGTACCAGCTGC  CCACCGT GCGGGG 1b  −7  
#4+  D5-5/18  −5 GTGGATACAGCTATG  AGGTGAAAAACCACCCCCCAAG AACACAGACCCACCCAGAGC  1a  +34  
#5  D2-2  −9 AGGATATTGTAGTAGTACCAGC  CCAGCCTTC CGGGG 1b  −2  
#6*+  D3-3  −8 GTATTACGATTTTTGGAGTGGTT  TAACCAACTCT CGCGGGG 1b  +1  
#7* D7-27  0 CTAACTGGGGA  CACCCTTACCTATA CCGCGGGG 1b  +2  
#8+  D3-9  −6  GTATTACGATATTTTGACTGGTTAT AAGATCCAGG GGGG 1b  −2  
#9*+ D2-2  −5  AGGATATTGTAGTAGTACCAGCTGCT TCCGACCCCTGTGATCTAC CCGCGGGG 1b +4  
#10*+  D5-5/18  −2  GTGGATACAGCTATGGTT CCTTTTCGGTGGCCAACCGACAG GGGG 1b 0  
#11  D3-10  −12  GTATTACTATGGTTCGGGG   GGGG 1b  0  
#12  D3-3  −3 GTATTACGATTTTTGGAGTGGTTATTAT  TGTTCGGCCAAATAC GGG 1b  +1  
#13+  D3-9  −7 GTATTACGATATTTTGACTGGTTA  GCAATTCGGGGATTGGTAATGAGAAA GCGGGG 1b  +4  
#14  D3-3  −7 GTATTACGATTTTTGGAGTGGTTA CCTGGCGCCGCTATTCGGTAGGCGGACCCAAAAAGATAAGGGCCCCGACGAGTTTGCATGA GCTGTCCTTCAGGGTCTTCCTGAAATG  2a  −9  
#15  D4-4 −7  TGACTACAG   GTCTTCCTGAAATG  2a  +8  
#16 D5-5/18  −1  GTGGATACAGCTATGGTTA  TTGGCCT GCTCC 2b  −11  
#17* D2-8  −3 AGGATATTGTACTAATGGTGTATGCTAT  GTAGGGG TCC 2b  −5  
#18  D2-15  −1 ATTGTAGTGGTGGTAGCTGCTACTC  GTGTACTACTCTTGGGGGGGC GAAATG  2a  +17  
#19  D2-2  −12 AGGATATTGTAGTAGTACC  GGGTCGGTAGGG AAGCAGGAAACCTGTGGTATGAAGC  3a  −14  
#20  D4-17 −1  TGACTACGGTGACTA  GCCTCCCTGCCATACCAACGCCCTTC CGCTCC 2b  0  
#21  D2-15  −19 AGGATATTGTAG  CCCAAGCATAGGGTG CCAAGAAAGCAGGAAACCTGTGGTAT  3a  −7  
#22*+  D2-21 −10  AGCATATTGTGGTGGTGA  TTTGAAGCAGGGGGGCTT CTCC 2b  −2  
#23+  D3-3  −4 GTATTACGATTTTTGGAGTGGTTATTA CGGGTCGGTCCGAACCGCAACAGGGGGTTCTTTGTC CC 2b  −4  
#24  D2-2  −11  AGGATATTGTAGTAGTACCA CCG CGCTCC 2b  0  
#25+ D1-26  −5  GGTATAGTGGGAGCT  CTACAAGGGGACTCTCCC CTCC 2b  −2  
#26+  D4-4  0 TGACTACAGTAACTAC  TTTC CGCTCC 2b 0  
#27+  D2-2  −4  AGGATATTGTAGTAGTACCAGCTGCTA CCCAACCCCATG ACGCTCC 2b  +1 
#28  D3-3  −32  cactgt TTCGGAAGTTGTGCCAACGACCACA GCTCC 2b 0  
#29  D3-10  −6  GTATTACTATGGTTCGGGGAGTTAT CGTGTGGGTTTTGGGAGACGCAGTCCC  CC 2b −3  
#30+  D1-7  0  GGTATAACTGGAACTAC CTCACCTTCC TCC 2b  −2  
#31+ D3-9  −6  GTATTACGATATTTTGACTGGTTAT  AACCTCTTAAG GTGGTATGAAGC  3a  −10  
#32  D2-2  −3 AGGATATTGTAGTAGTACCAGCTGCTAT  TTGTCTTTGTTACCTCTTTCATTTT TGTGGTATGAAGC  3a  −6  
#33*+  D2-2  −8 AGGATATTGTAGTAGTACCAGCT  TCGGTCC ACCTGTGGTATGAAGC  3a  −2  
#34  D2-2  −8  AGGATATTGTAGTAGTACCAGCT  CC 2b  +18  
#35*+  D3-3  −6 GTATTACGATTTTTGGAGTGGTTAT  CAGGGTAG GGGCCTCAGGGAACAGAATGATCAGAC  3b  −12  
#36  D3-3 −3  GTATTACGATTTTTGGAGTGGTTATTAT  TTCGATCACGCCGG TGATCAGAC  3b  −22  
#37  D3-16  −3 TTACGTTTGGGGGAGTTATCGTTAT  GTGGTCCCTTCCCGCTATA GGCGGGCCTCAGGGAACAGAATGATCAG  3b  0  
#38*+ JH6sp   ggtttttgtggggtgaggatggaca CCCTACAACGCGCGCACAAGACGT GCGGGCCTCAGGGAACAGAATGATCAG  3b  +4  
#39 D3-9  −7  GTATTACGATATTTTGACTGGTTA  CGGGCTTCC GAATGATCAGAC  3b  −13  
#40  D3-10  −6 GTATTACTATGGTTCGGGGAGTTAT  GTTTG GCAGGAAACCTGTGGTATGAAGC  3a  +56 
*

Samples marked with an asterisk (*) were PCR-amplified and sequenced twice. Samples marked by a plus (+) were amplified with a 4:1 proportion of Taq DNA polymerase and Vent high-fidelity DNA polymerase.

†

S: status of coding end or mbr end processing (0 = precise, −n = deletion, +n = duplication, P + n = P nucleotide).

‡

De novo nucleotide additions are represented in bold character, DH segments found in the direct breakpoints are shown between brackets. Mutations in the DH sequences are underlined. These sequence data are available from Genbank under accession numbers AF147979 to AF148063.

F2-153

P nucleotides are shown in italics.

F2-155

For practical purposes, the mbr sequence was broken into 6 immediately adjacent pieces (1a to 3B). Clusters 1, 2, and 3 are underlined.

View Large
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal