Ligand binding affinities of wild-type and mutated GM-CSF receptors expressed in COS cells
| Mutant . | kd (mean ± SD) . | Receptor affinity . |
|---|---|---|
| Wild-type GMRαβ | 203.63 ± 58.03 pmol/L | High affinity |
| Wild-type GMRα | 6.38 ± 1.57 nmol/L | Low affinity |
| GMRβ-Ala58 | 8.41 ± 1.88 nmol/L | Low affinity |
| GMRβ-Ala191 | 8.90 ± 1.99 nmol/L | Low affinity |
| GMRβ-Ala346 | 5.74 ± 1.65 nmol/L | Low affinity |
| GMRβ-Ala58/191/346 | 7.10 ± 1.62 nmol/L | Low affinity |
| GMRβ-Asp58 | 7.68 ± 2.27 nmol/L | Low affinity |
| GMRβ-Asp191 | 6.69 ± 1.10 nmol/L | Low affinity |
| GMRβ-Asp346 | 6.89 ± 1.87 nmol/L | Low affinity |
| GMRβ-Asp58/191/346 | 7.59 ± 3.03 nmol/L | Low affinity |
| Mutant . | kd (mean ± SD) . | Receptor affinity . |
|---|---|---|
| Wild-type GMRαβ | 203.63 ± 58.03 pmol/L | High affinity |
| Wild-type GMRα | 6.38 ± 1.57 nmol/L | Low affinity |
| GMRβ-Ala58 | 8.41 ± 1.88 nmol/L | Low affinity |
| GMRβ-Ala191 | 8.90 ± 1.99 nmol/L | Low affinity |
| GMRβ-Ala346 | 5.74 ± 1.65 nmol/L | Low affinity |
| GMRβ-Ala58/191/346 | 7.10 ± 1.62 nmol/L | Low affinity |
| GMRβ-Asp58 | 7.68 ± 2.27 nmol/L | Low affinity |
| GMRβ-Asp191 | 6.69 ± 1.10 nmol/L | Low affinity |
| GMRβ-Asp346 | 6.89 ± 1.87 nmol/L | Low affinity |
| GMRβ-Asp58/191/346 | 7.59 ± 3.03 nmol/L | Low affinity |
Expression plasmids encoding the α subunit were transfected alone or cotransfected with plasmids encoding wild-type or mutated β subunit to measure GM-CSF binding affinity. The data were derived from at least 3 independent experiments.