Table 4.

Calculated association and dissociation constants of the interaction of anti-FVIII IgG with C2 and A2 domains, light chain of FVIII, and intact FVIII

Source of IgG Epitope location ka (105 M−1 s−1)kd (ms−1) Kd (nM)
Alloantibody HA  C2  1.99  2.45  12.30  
 LC 1.58  0.50  3.17  
 A2  0.010  0.046  4.65 
 FVIII  1.32  0.47  3.56  
Autoantibody  C2 27.40  5.81  2.12  
 LC  1.28  0.62  4.86 
 A2  0.129  0.099  7.68  
 FVIII  1.25  0.61 4.94  
Nonresponder HA  C2  0.57  0.60  1.05 
 LC  1.31  0.52  3.99  
 A2  0.012  0.009 8.14  
 FVIII  6.71  1.13  1.68  
Healthy donor C2  4.55  3.83  8.41  
 LC  1.18  0.57  4.83 
 A2  0.014  0.009  6.80  
 FVIII  0.67  2.61 38.7  
IVIg  C2  0.66  3.58  53.5  
 LC  2.22 0.71  3.18  
 A2  0.095  0.102  10.72 
 FVIII  1.96  0.74  3.76 
Source of IgG Epitope location ka (105 M−1 s−1)kd (ms−1) Kd (nM)
Alloantibody HA  C2  1.99  2.45  12.30  
 LC 1.58  0.50  3.17  
 A2  0.010  0.046  4.65 
 FVIII  1.32  0.47  3.56  
Autoantibody  C2 27.40  5.81  2.12  
 LC  1.28  0.62  4.86 
 A2  0.129  0.099  7.68  
 FVIII  1.25  0.61 4.94  
Nonresponder HA  C2  0.57  0.60  1.05 
 LC  1.31  0.52  3.99  
 A2  0.012  0.009 8.14  
 FVIII  6.71  1.13  1.68  
Healthy donor C2  4.55  3.83  8.41  
 LC  1.18  0.57  4.83 
 A2  0.014  0.009  6.80  
 FVIII  0.67  2.61 38.7  
IVIg  C2  0.66  3.58  53.5  
 LC  2.22 0.71  3.18  
 A2  0.095  0.102  10.72 
 FVIII  1.96  0.74  3.76 

Association and dissociation constants of the interaction of anti-FVIII IgG with the A2 and C2 domains, light chain of FVIII, and with intact FVIII were calculated from the binding data as described in “Patients and methods.” See Table 1 for description of IgG sources.

ka: association constant; kd: dissociation constant; Kd: equilibrium dissociation constant.

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