Immunoprecipitation titers of anti-FVIII antibodies with intact FVIII, light chain, and A2 and C2 domains of FVIII
| Source of IgG . | Immunoprecipitation titers (units/mg IgG) . | |||
|---|---|---|---|---|
| Recombinant FVIII . | Light chain . | A2 . | C2 . | |
| Alloantibody HA | 417 | 371 | 827 | 151 |
| Autoantibody | 158 | 700 | 29.4 | 229 |
| Nonresponder HA | 8.2 | 45.7 | 20.4 | 3.6 |
| 9.7 | 25.1 | 6.52 | 9 | |
| Healthy donor | 6.3 | 55.9 | 16.3 | 0 |
| IVIg | 274 | 1164 | 23 | 1388 |
| Source of IgG . | Immunoprecipitation titers (units/mg IgG) . | |||
|---|---|---|---|---|
| Recombinant FVIII . | Light chain . | A2 . | C2 . | |
| Alloantibody HA | 417 | 371 | 827 | 151 |
| Autoantibody | 158 | 700 | 29.4 | 229 |
| Nonresponder HA | 8.2 | 45.7 | 20.4 | 3.6 |
| 9.7 | 25.1 | 6.52 | 9 | |
| Healthy donor | 6.3 | 55.9 | 16.3 | 0 |
| IVIg | 274 | 1164 | 23 | 1388 |
Affinity-purified IgG was tested for its ability to immunoprecipitate recombinant FVIII and its purified fragments. Immunoprecipitating titers were calculated as described in “Patients and methods.” See Table 1 for description of IgG sources.