Table 1. Heparin preparations used for... | American Society of Hematology

Table 1.

Heparin preparations used for rosette disruption assays on FCR3S1.2 parasites

Preparation^*	Sulfate groups/ disaccharide unit^†	IC₅₀ of rosette disruption^‡
Native	2.7	8 μg/mL
N-desulfated/N-acetylated	1.6	>1000 μg/mL
2-O-desulfated	1.9	80 μg/mL
6-O-desulfated	1.7	90 μg/mL
N-/2-O-desulfated	1.1	ne^1-153
N-/6-O-desulfated	0.7	ne
2-O-/6-O-desulfated	1.3	105 μg/mL
N-/2-O-/6-O-desulfated	0.3	ne

Preparation^*	Sulfate groups/ disaccharide unit^†	IC₅₀ of rosette disruption^‡
Native	2.7	8 μg/mL
N-desulfated/N-acetylated	1.6	>1000 μg/mL
2-O-desulfated	1.9	80 μg/mL
6-O-desulfated	1.7	90 μg/mL
N-/2-O-desulfated	1.1	ne^1-153
N-/6-O-desulfated	0.7	ne
2-O-/6-O-desulfated	1.3	105 μg/mL
N-/2-O-/6-O-desulfated	0.3	ne

Bovine lung heparin was selectively modified as described.15

Average number of sulfated groups/single disaccharide unit as calculated from compositional analysis.

Concentration of component needed to achieve 50% disruption of rosettes.

ne, no effect.