DC were incubated with or without lipopolysaccharide (1 μg/mL) for 24 hours, washed, and cultured in medium in the absence or presence of anti-CD95 (CH11; 1 μg/mL) for 24 hours. The percentage of nonviable cells was determined with use of annexin V-FITC and PI staining and fluorescence-activated cell sorting. Data represent mean (±SEM) values from 5 independent experiments on samples from 5 different donors. There was no significant difference between values obtained with medium and those obtained with anti-CD95 or between values obtained with mature DC and those obtained with immature DC.