Cell-labeling results indicating facilitation by bisindolylmaleimide (Bis) of CD95-mediated apoptosis in human dendritic cells (DC)
| Treatment of DC . | Cell-labeling results (% DC)* . | PI+ . | Hypodiploid nuclei (%)† . | ||
|---|---|---|---|---|---|
| Annexin V− PI− . | Annexin V+ PI− . | Annexin V+ PI+ . | |||
| Medium | 87.2 ± 2.5 | 3.3 ± 0.7 | 7.8 ± 1.6 | 1.6 ± 0.2 | 3.8 ± 0.8 |
| Anti-CD95 | 85.8 ± 2.8 | 3.7 ± 0.8 | 8.6 ± 1.8 | 1.9 ± 0.4 | 8.8 ± 2.4 |
| Bis | 84.7 ± 4.3 | 6.3 ± 1.3 | 7.4 ± 2.6 | 1.6 ± 0.5 | 9.6 ± 1.9 |
| Bis + Anti-CD95 | 52.6 ± 1.4 | 12.4 ± 2.6‡ | 22.4 ± 1.3‡ | 12.4 ± 3.0‡ | 46.1 ± 6.71-153 |
| Treatment of DC . | Cell-labeling results (% DC)* . | PI+ . | Hypodiploid nuclei (%)† . | ||
|---|---|---|---|---|---|
| Annexin V− PI− . | Annexin V+ PI− . | Annexin V+ PI+ . | |||
| Medium | 87.2 ± 2.5 | 3.3 ± 0.7 | 7.8 ± 1.6 | 1.6 ± 0.2 | 3.8 ± 0.8 |
| Anti-CD95 | 85.8 ± 2.8 | 3.7 ± 0.8 | 8.6 ± 1.8 | 1.9 ± 0.4 | 8.8 ± 2.4 |
| Bis | 84.7 ± 4.3 | 6.3 ± 1.3 | 7.4 ± 2.6 | 1.6 ± 0.5 | 9.6 ± 1.9 |
| Bis + Anti-CD95 | 52.6 ± 1.4 | 12.4 ± 2.6‡ | 22.4 ± 1.3‡ | 12.4 ± 3.0‡ | 46.1 ± 6.71-153 |
DC were incubated for 18 hours in medium alone or in the presence of either CH-11 anti-CD95 monoclonal antibody (1 μg/mL) or Bis III (20 μmol/L).
DC were stained with fluorescein isothiocyanate-conjugated (FITC) annexin V followed by propidium iodide (PI) and analyzed with use of flow cytometry. Data represent mean (±SEM) percentage of cells in each quadrant of dot-plot analysis from 6 independent experiments on samples from 6 different donors.
P < .05 compared with DC incubated in medium alone (Wilcoxon two-tailed test).
DC were fixed before incubation with PI and RNase. Data represent mean (±SEM) percentage of hypodiploid nuclei measured by flow cytometry in 9 independent experiments on samples from 9 different donors.
P < .005 compared with DC incubated in medium alone (Wilcoxon two-tailed test).