Table 1. Kinetic parameters of the PDE3A... | American Society of Hematology

Table 1.

Kinetic parameters of the PDE3A mutants

	Expression (%)	K_m μmol/L (mean ± SD)	k_cat s⁻¹ (×100) (mean ± SD)	k_cat/K_m μmol/L⁻¹ s⁻¹	ΔΔGT K_cal/mol
PDE3AΔ1	100	0.21 ± 0.023	185.72 ± 23.55	8.759
H752A	12	0.22 ± 0.029	0.14 ± 0.02^*	0.007	4.32
H756A	15	0.21 ± 0.034	0.08 ± 0.02^*	0.004	4.66
E825A	24	0.193 ± 0.015	4.75 ± 0.88^*	0.246	2.10
H836A	16	0.18 ± 0.185	0.97 ± 0.27^*	0.054	3.05
E866A	34	2.35 ± 0.308^*	23.58 ± 4.32^*	0.099	2.71
E971A	14	1.08 ± 0.253^*	2.06 ± 0.024^*	0.019	3.69

	Expression (%)	K_m μmol/L (mean ± SD)	k_cat s⁻¹ (×100) (mean ± SD)	k_cat/K_m μmol/L⁻¹ s⁻¹	ΔΔGT K_cal/mol
PDE3AΔ1	100	0.21 ± 0.023	185.72 ± 23.55	8.759
H752A	12	0.22 ± 0.029	0.14 ± 0.02^*	0.007	4.32
H756A	15	0.21 ± 0.034	0.08 ± 0.02^*	0.004	4.66
E825A	24	0.193 ± 0.015	4.75 ± 0.88^*	0.246	2.10
H836A	16	0.18 ± 0.185	0.97 ± 0.27^*	0.054	3.05
E866A	34	2.35 ± 0.308^*	23.58 ± 4.32^*	0.099	2.71
E971A	14	1.08 ± 0.253^*	2.06 ± 0.024^*	0.019	3.69

Mutants are listed in order of their position in the sequence. Results represent the mean of 3 determinations ± SD.

Statistical analyses were performed using Student t-test. Each mutant was compared to PDE3AΔ1.

P < .01.