Reduction of frequency of CFU–IL-7 in op/opbone marrow
| Factor addition . | No. of colonies per 105 cells . | |
|---|---|---|
| +/? . | op/op . | |
| Experiment no. 1 | ||
| Medium | 0 | 0 |
| IL-3 | 295.0 ± 43.3 | 363.3 ± 53.9 |
| IL-7 | 48.3 ± 11.5 | 1.7 ± 2.9* |
| Experiment no. 2 | ||
| Medium | 0 | 0 |
| IL-3 | 241.5 ± 33.5 | 230.0 ± 73.5 |
| IL-7 | 68.5 ± 12.5 | 8.3 ± 7.5* |
| Experiment no. 3 | ||
| Medium | 0 | 0 |
| IL-3 | 655.0 ± 13.2 | 335.0 ± 32.8* |
| GM-CSF | 435.0 ± 73.7 | 443.0 ± 92.5 |
| IL-7 | 76.7 ± 15.3 | 0* |
| Factor addition . | No. of colonies per 105 cells . | |
|---|---|---|
| +/? . | op/op . | |
| Experiment no. 1 | ||
| Medium | 0 | 0 |
| IL-3 | 295.0 ± 43.3 | 363.3 ± 53.9 |
| IL-7 | 48.3 ± 11.5 | 1.7 ± 2.9* |
| Experiment no. 2 | ||
| Medium | 0 | 0 |
| IL-3 | 241.5 ± 33.5 | 230.0 ± 73.5 |
| IL-7 | 68.5 ± 12.5 | 8.3 ± 7.5* |
| Experiment no. 3 | ||
| Medium | 0 | 0 |
| IL-3 | 655.0 ± 13.2 | 335.0 ± 32.8* |
| GM-CSF | 435.0 ± 73.7 | 443.0 ± 92.5 |
| IL-7 | 76.7 ± 15.3 | 0* |
Freshly prepared bone marrow cells from 8-week-old op/ophomozygotes and their normal littermates were cultured in methylcellulose semi-solid medium with IL-3, GM-CSF, or IL-7 for 7 days. Data are expressed as the mean ± SD of triplicate cultures.
Significantly different from the corresponding normal mouse cultures (P < .002).