Leukocyte antibody analysis and effects on PMN responses in transfused patients
| Patient . | No.transfusions . | PMN dose (cells ×109) . | Positive XM* . | PRA baseline/ max/end . | GIFT baseline/ end . | LIFT baseline/ end . | PMN 1-hr inc (cells ×103/μL) . | Next AM PMN (cells ×103/μL) . | Oral PMN (cells ×106) baseline/post-Tx . |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 96.5 ± 46.6 | 1/1 | 83/83/3 | +/+ | +++/+++ | 5.3 ± 1.4 | 15 | 0/0.01 |
| 2 | 11 | 76.5 ± 26.5 | 11/11 | 7/93/93 | −/− | −/− | 2.7 ± 1.6 | 2.7 ± 1.7 | 0.01/0.22 |
| 3 | 11 | 91.2 ± 22.1 | 0/11 | 0/0/0 | −/− | −/− | 0.2 ± 0.1 | 0.0 ± 0.0 | 0/0 |
| 4 | 14 | 79.2 ± 14.7 | 0/14 | 0/0/0 | −/− | −/− | 3.1 ± 1.5 | 1.4 ± 1.0 | 0.02/0.07 |
| 5 | 3 | 79 ± 10.1 | 1/3 | 0/0/na | +/+ | −/− | 1.8 ± 1.0 | 1.5 ± 1.5 | 0.02/0.72 |
| 6 | 1 | 81.6 | 0/1 | 0/0/na | −/NA | −/NA | NA | 8.9 | NA |
| 7 | 13 | 80.3 ± 17.8 | 0/13 | 7/7/0 | −/− | −/− | 1.8 ± 1.4 | 0.9 ± 1.1 | 0.05/0.22 |
| 8 | 3 | 80.6 ± 16.2 | 0/3 | 0/0/0 | −/− | −/− | 1.3 ± 0.8 | 0.6 ± 0.8 | 0/0.02 |
| 9 | 7 | 85.5 ± 14.7 | 1/7 | 0/22/3 | −/+ | −/− | 1.4 ± 1.6 | 0.9 ± 0.4 | NA |
| 10 | 8 | 59.7 ± 30.1 | 0/8 | 0/0/0 | +/− | −/− | 4.9 ± 4.2 | 4.3 ± 2.5 | 0.05/1.04 |
| 11 | 7 | 105.0 ± 17.8 | 0/7 | 0/0/0 | −/− | −/− | 0.9 ± 1.0 | 1.5 ± 1.2 | NA |
| 12 | 9 | 82.9 ± 17.3 | 1/9 | 18/18/13 | +++/+++ | +++/+++ | 0.2 ± 0.4 | 0.3 ± 0.2 | 0.02/0.03 |
| 13 | 18 | 89.2 ± 26.3 | 3/18 | 0/24/24 | −/+ | −/+ | 5.1 ± 4.3 | 6.9 ± 3.3 | 0/0.3 |
| 14 | 2 | 62.0 ± 17.7 | 0/2 | 0/0/0 | −/NA | −/NA | NA | NA | NA |
| 15 | 25 | 78.2 ± 27.3 | 0/25 | 0/3/0 | −/− | −/− | 2.7 ± 1.3 | 3.3 ± 1.4 | 0/0.09 |
| 16 | 2 | 106.6 ± 32.5 | 1/2 | 0/95/95 | −/+ | −/− | 1.0 ± 0.1 | 3.7 ± 3.6 | NA/1.84 |
| 17 | 16 | 79.0 ± 21.1 | 6/16 | 6/29/6 | +/− | +/− | 4.5 ± 1.9 | 4.0 ± 2.2 | NA/4.26 |
| 19 | 12 | 69.7 ± 25.5 | 0/11 | 0/3/0 | −/− | −/− | 1.3 ± 0.9 | 0.8 ± 0.9 | 0/0.53 |
| 20 | 1 | 50.8 | 0/1 | 0/0/0 | +/NA | −/NA | NA | NA | NA |
| Group mean (±SD) | 8.6 | 2.58 ± 2.60 | 2.62 ± 2.83 | 0.01/0.52 | |||||
| Normal12 | 0.52 |
| Patient . | No.transfusions . | PMN dose (cells ×109) . | Positive XM* . | PRA baseline/ max/end . | GIFT baseline/ end . | LIFT baseline/ end . | PMN 1-hr inc (cells ×103/μL) . | Next AM PMN (cells ×103/μL) . | Oral PMN (cells ×106) baseline/post-Tx . |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 96.5 ± 46.6 | 1/1 | 83/83/3 | +/+ | +++/+++ | 5.3 ± 1.4 | 15 | 0/0.01 |
| 2 | 11 | 76.5 ± 26.5 | 11/11 | 7/93/93 | −/− | −/− | 2.7 ± 1.6 | 2.7 ± 1.7 | 0.01/0.22 |
| 3 | 11 | 91.2 ± 22.1 | 0/11 | 0/0/0 | −/− | −/− | 0.2 ± 0.1 | 0.0 ± 0.0 | 0/0 |
| 4 | 14 | 79.2 ± 14.7 | 0/14 | 0/0/0 | −/− | −/− | 3.1 ± 1.5 | 1.4 ± 1.0 | 0.02/0.07 |
| 5 | 3 | 79 ± 10.1 | 1/3 | 0/0/na | +/+ | −/− | 1.8 ± 1.0 | 1.5 ± 1.5 | 0.02/0.72 |
| 6 | 1 | 81.6 | 0/1 | 0/0/na | −/NA | −/NA | NA | 8.9 | NA |
| 7 | 13 | 80.3 ± 17.8 | 0/13 | 7/7/0 | −/− | −/− | 1.8 ± 1.4 | 0.9 ± 1.1 | 0.05/0.22 |
| 8 | 3 | 80.6 ± 16.2 | 0/3 | 0/0/0 | −/− | −/− | 1.3 ± 0.8 | 0.6 ± 0.8 | 0/0.02 |
| 9 | 7 | 85.5 ± 14.7 | 1/7 | 0/22/3 | −/+ | −/− | 1.4 ± 1.6 | 0.9 ± 0.4 | NA |
| 10 | 8 | 59.7 ± 30.1 | 0/8 | 0/0/0 | +/− | −/− | 4.9 ± 4.2 | 4.3 ± 2.5 | 0.05/1.04 |
| 11 | 7 | 105.0 ± 17.8 | 0/7 | 0/0/0 | −/− | −/− | 0.9 ± 1.0 | 1.5 ± 1.2 | NA |
| 12 | 9 | 82.9 ± 17.3 | 1/9 | 18/18/13 | +++/+++ | +++/+++ | 0.2 ± 0.4 | 0.3 ± 0.2 | 0.02/0.03 |
| 13 | 18 | 89.2 ± 26.3 | 3/18 | 0/24/24 | −/+ | −/+ | 5.1 ± 4.3 | 6.9 ± 3.3 | 0/0.3 |
| 14 | 2 | 62.0 ± 17.7 | 0/2 | 0/0/0 | −/NA | −/NA | NA | NA | NA |
| 15 | 25 | 78.2 ± 27.3 | 0/25 | 0/3/0 | −/− | −/− | 2.7 ± 1.3 | 3.3 ± 1.4 | 0/0.09 |
| 16 | 2 | 106.6 ± 32.5 | 1/2 | 0/95/95 | −/+ | −/− | 1.0 ± 0.1 | 3.7 ± 3.6 | NA/1.84 |
| 17 | 16 | 79.0 ± 21.1 | 6/16 | 6/29/6 | +/− | +/− | 4.5 ± 1.9 | 4.0 ± 2.2 | NA/4.26 |
| 19 | 12 | 69.7 ± 25.5 | 0/11 | 0/3/0 | −/− | −/− | 1.3 ± 0.9 | 0.8 ± 0.9 | 0/0.53 |
| 20 | 1 | 50.8 | 0/1 | 0/0/0 | +/NA | −/NA | NA | NA | NA |
| Group mean (±SD) | 8.6 | 2.58 ± 2.60 | 2.62 ± 2.83 | 0.01/0.52 | |||||
| Normal12 | 0.52 |
PMN indicates polymorphonuclear leukocyte; PRA, positive cross-match; GIFT, granulocyte immunofluorescence test; LIFT, lymphocyte immunofluorescence test; inc, increments; Tx, treatment; NA, not available.
XM = T-cell cross-match (see text): positive cross-matches/total donors tested.