Table. HBG activity in organs after treatment and effect of treatment on GAG levels in liver and spleen
| Genotype . | Transplanted macrophages . | Days after infusion . | HBG activity in organs . | GAG level in liver . | GAG level in spleen . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Liver . | Spleen . | CS . | DS . | HA . | CS . | DS . | HA . | |||
| +/−(n = 5) | — | — | 1030 ± 73 | 1369 ± 459 | 0.05 ± 0.01 | 0.04 ± 0.01 | 0.01 ± 0.00 | 0.68 ± 0.19 | 0.14 ± 0.06 | 0.06 ± 0.02 |
| −/−(n = 5) | — | — | 0.84 ± 0.75 | 0.44 ± 0.39 | 1.92 ± 0.78 | 0.39 ± 0.07 | 2.00 ± 0.29 | 5.78 ± 2.70 | 1.36 ± 0.53 | 2.21 ± 1.00 |
| −/−(n = 4) | Normal | 7 | 32.9 ± 8.6 | 35.0 ± 22.3 | nd | nd | nd | nd | nd | nd |
| −/−(n = 3) | Normal | 38 | 3.6 ± 1.5 | 2.3 ± 0.6 | 0.37 ± 0.09* | 0.07 ± 0.01* | 0.14 ± 0.06* | 3.64 ± 1.04 | 0.54 ± 0.13* | 0.28 ± 0.13 |
| −/−(n = 3) | Transduced | 7 | 28.5 ± 4.3 | 32.4 ± 7.3 | nd | nd | nd | nd | nd | nd |
| −/−(n = 3) | Transduced | 38 | 3.9 ± 0.8 | 2.3 ± 0.8 | 0.41 ± 0.10 | 0.14 ± 0.03* | 0.20 ± 0.07* | 2.80 ± 0.50 | 0.53 ± 0.12* | 0.24 ± 0.09* |
| Genotype . | Transplanted macrophages . | Days after infusion . | HBG activity in organs . | GAG level in liver . | GAG level in spleen . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Liver . | Spleen . | CS . | DS . | HA . | CS . | DS . | HA . | |||
| +/−(n = 5) | — | — | 1030 ± 73 | 1369 ± 459 | 0.05 ± 0.01 | 0.04 ± 0.01 | 0.01 ± 0.00 | 0.68 ± 0.19 | 0.14 ± 0.06 | 0.06 ± 0.02 |
| −/−(n = 5) | — | — | 0.84 ± 0.75 | 0.44 ± 0.39 | 1.92 ± 0.78 | 0.39 ± 0.07 | 2.00 ± 0.29 | 5.78 ± 2.70 | 1.36 ± 0.53 | 2.21 ± 1.00 |
| −/−(n = 4) | Normal | 7 | 32.9 ± 8.6 | 35.0 ± 22.3 | nd | nd | nd | nd | nd | nd |
| −/−(n = 3) | Normal | 38 | 3.6 ± 1.5 | 2.3 ± 0.6 | 0.37 ± 0.09* | 0.07 ± 0.01* | 0.14 ± 0.06* | 3.64 ± 1.04 | 0.54 ± 0.13* | 0.28 ± 0.13 |
| −/−(n = 3) | Transduced | 7 | 28.5 ± 4.3 | 32.4 ± 7.3 | nd | nd | nd | nd | nd | nd |
| −/−(n = 3) | Transduced | 38 | 3.9 ± 0.8 | 2.3 ± 0.8 | 0.41 ± 0.10 | 0.14 ± 0.03* | 0.20 ± 0.07* | 2.80 ± 0.50 | 0.53 ± 0.12* | 0.24 ± 0.09* |
HBG activity is expressed as nmol/h/mg. GAGs levels in liver and spleen are expressed in μg/mg of wet tissue. The data presented are mean ± SD.
CS, chondroitin sulfate; DS, dermatan sulfate; HA, hyaluronic acid; nd, not determined.
Indicates significant reduction (P < .05, 2-samplet test) of GAGs in tissues.