Oligonucleotide probes for allele-specific oligonucleotide hybridization
| Mutation . | Normal probe (wash temp) . | Mutant probe (wash temp) . |
|---|---|---|
| 1456 C→T | 5′-TTCTGTCTCGGTACCGA-3′(50C) | 5′-TTCTGTCTTGGTACCGA-3′(48C) |
| 1468 C→T | 5′-TACCGACCTCGGGCAG-3′(52C) | 5′-TACCGACCTTGGGCAG-3′(50C) |
| 1484 C→T | 5′-AGTCATTGCTGTCACCC-3′(52C) | 5′-AGTCATTGTTGTCACCC-3′(50C) |
| 1529 G→A | 5′-CTTATGCCGAGGAGTCT-3′(50C) | 5′-CTTATGCCAAGGAGTCT-3′(48C) |
| Mutation . | Normal probe (wash temp) . | Mutant probe (wash temp) . |
|---|---|---|
| 1456 C→T | 5′-TTCTGTCTCGGTACCGA-3′(50C) | 5′-TTCTGTCTTGGTACCGA-3′(48C) |
| 1468 C→T | 5′-TACCGACCTCGGGCAG-3′(52C) | 5′-TACCGACCTTGGGCAG-3′(50C) |
| 1484 C→T | 5′-AGTCATTGCTGTCACCC-3′(52C) | 5′-AGTCATTGTTGTCACCC-3′(50C) |
| 1529 G→A | 5′-CTTATGCCGAGGAGTCT-3′(50C) | 5′-CTTATGCCAAGGAGTCT-3′(48C) |
The nucleotides that are varied are shown in underlined bold font.