Generation of Naive and Memory T Cells in IUT Versus TBI-Conditioned Recipients
| Group . | Spleen . | Lymph Node . | ||||||
|---|---|---|---|---|---|---|---|---|
| CD44 . | CD8 . | CD4 . | CD8 . | |||||
| No. . | CD44 . | No. . | CD44 . | No. . | CD44 . | No. . | CD44 . | |
| IUT NTCD | 21 (3) | 435 (43) | 17 (2) | 524 (46) | 3 (2) | 398 (48) | 2 (1) | 357 (33) |
| TBI TCD | 16 (3) | 587 (32)* | 9 (1)* | 780 (49)* | 7 (1) | 545 (15)* | 4 (1) | 612 (17)* |
| Controls | 21 (3) | 436 (19) | 14 (2) | 470 (37) | 7 (1) | 360 (90) | 5 (1) | 398 (93) |
| Group . | Spleen . | Lymph Node . | ||||||
|---|---|---|---|---|---|---|---|---|
| CD44 . | CD8 . | CD4 . | CD8 . | |||||
| No. . | CD44 . | No. . | CD44 . | No. . | CD44 . | No. . | CD44 . | |
| IUT NTCD | 21 (3) | 435 (43) | 17 (2) | 524 (46) | 3 (2) | 398 (48) | 2 (1) | 357 (33) |
| TBI TCD | 16 (3) | 587 (32)* | 9 (1)* | 780 (49)* | 7 (1) | 545 (15)* | 4 (1) | 612 (17)* |
| Controls | 21 (3) | 436 (19) | 14 (2) | 470 (37) | 7 (1) | 360 (90) | 5 (1) | 398 (93) |
Spleen and LN from 5 high-level chimeras per group were individually analyzed for CD4+ and CD8+ T-cell content. The absolute numbers (No.) × 10−6 are shown. CD4+ and CD8+ T cells were analyzed by FACS for the expression of CD44 as an indicator of memory cell generation. The mean channel fluorescence data are shown. The standard error of the mean is shown in parentheses.
A significant (P < .05) difference between IUT NTCD and other groups.