Effect of Transfected Thrombin Receptor PAR-1 on Pulmonary Metastasis of B16F10 Murine Tumor Cells
| N . | Group . | Median Nodule No. (range) . | Median Nodule Volume (μL; range) . | Median Tumor Mass (μL) . | Fold Increase* . | P† . |
|---|---|---|---|---|---|---|
| 20 | Mock | 1.0 (0-5) | 0.35 (0-5) | 0.35 | ||
| 20 | Mock + T | 8.3 (0-13) | 11.2 (2-71) | 93 | 266 | .005 |
| 10 | AS | 2 (0-5) | 1.1 (0-4) | 2.2 | ||
| 10 | AS + T | 2 (0-5) | 0.75 (0-4) | 1.5 | ||
| 10 | Sense 6 | 0 (1-8) | 0 (0-7) | 0 | ||
| 10 | Sense 6 + T | 6.5 (2-15) | 10.7 (0.1-43) | 70 | 200 | .02 |
| 20 | Sense 14 | 0 (0-4) | 0 (0-10) | 0 | ||
| 20 | Sense 14 + T | 13.8 (2-23) | 31.1 (1-192) | 429 | 1,226 | .0001 |
| N . | Group . | Median Nodule No. (range) . | Median Nodule Volume (μL; range) . | Median Tumor Mass (μL) . | Fold Increase* . | P† . |
|---|---|---|---|---|---|---|
| 20 | Mock | 1.0 (0-5) | 0.35 (0-5) | 0.35 | ||
| 20 | Mock + T | 8.3 (0-13) | 11.2 (2-71) | 93 | 266 | .005 |
| 10 | AS | 2 (0-5) | 1.1 (0-4) | 2.2 | ||
| 10 | AS + T | 2 (0-5) | 0.75 (0-4) | 1.5 | ||
| 10 | Sense 6 | 0 (1-8) | 0 (0-7) | 0 | ||
| 10 | Sense 6 + T | 6.5 (2-15) | 10.7 (0.1-43) | 70 | 200 | .02 |
| 20 | Sense 14 | 0 (0-4) | 0 (0-10) | 0 | ||
| 20 | Sense 14 + T | 13.8 (2-23) | 31.1 (1-192) | 429 | 1,226 | .0001 |
Experimental details are the same as in Table 1, with pulmonary metastasis examined on day 21.
Abbreviations: Mock, vector alone; T, thrombin (1 U/mL); AS, antisense 12 transfectant.
Increase compared with control mock transfectant.
P value refers to respective thrombin-induced increases between mock or sense transfections determined by Wilcoxon Rank Sum test.