Fifty Percent Inhibitory Concentrations of Inhibitors on IIbβ3 Activation
| Inhibitor . | Agonist . | |||
|---|---|---|---|---|
| ADP (20 μmol/L) . | α-Thrombin (0.1 U/mL) . | PMA (200 nmol/L) . | ||
| Before . | After* . | |||
| DCB | 1.4 ± 0.8 | 2.2 ± 0.3 | 5.4 ± 0.48 | 7.8 ± 1.0 |
| bepridil | 19 ± 7 | 42 ± 2 | 65 ± 12 | 13 ± 2 |
| Ro31-8220 | No inhibition | 0.064 ± 0.010 | ND | <0.06 |
| EIPA | 20 ± 5 | No inhibition | ND | ND |
| Inhibitor . | Agonist . | |||
|---|---|---|---|---|
| ADP (20 μmol/L) . | α-Thrombin (0.1 U/mL) . | PMA (200 nmol/L) . | ||
| Before . | After* . | |||
| DCB | 1.4 ± 0.8 | 2.2 ± 0.3 | 5.4 ± 0.48 | 7.8 ± 1.0 |
| bepridil | 19 ± 7 | 42 ± 2 | 65 ± 12 | 13 ± 2 |
| Ro31-8220 | No inhibition | 0.064 ± 0.010 | ND | <0.06 |
| EIPA | 20 ± 5 | No inhibition | ND | ND |
Experiments were performed as described in the legend of Fig 2 and 50% inhibitory concentration (IC50; μmol/L) was determined. Results are the mean ± SD of three separate experiments.
Abbreviation: ND, not determined.
Each inhibitor was added after thrombin stimulation.