Cell Lines Used in the Studies
| Cell Lines . | Expression . | Selection . | References . |
|---|---|---|---|
| LαβIXV | GP Ibα, GP Ibβ, GP IX, GPV | *HAT and Hygromycin | 16, 40 |
| LαβIX | GP Ibα, GP Ibβ, GP IX | HAT | 15 |
| LβIX | GP Ibβ, GP IX | G418 and Methotrexate | 15 |
| CHOαβIX | GP Ibα, GP Ibβ, GP IX | G418 | 15 |
| CHOβIX | GP Ibβ, GP IX | G418 and Methotrexate | 15 |
| Cell Lines . | Expression . | Selection . | References . |
|---|---|---|---|
| LαβIXV | GP Ibα, GP Ibβ, GP IX, GPV | *HAT and Hygromycin | 16, 40 |
| LαβIX | GP Ibα, GP Ibβ, GP IX | HAT | 15 |
| LβIX | GP Ibβ, GP IX | G418 and Methotrexate | 15 |
| CHOαβIX | GP Ibα, GP Ibβ, GP IX | G418 | 15 |
| CHOβIX | GP Ibβ, GP IX | G418 and Methotrexate | 15 |
HAT supplement (GIBCO) contains, at final concentrations, 100 μmol/L sodium hypoxanthin, 400 nmol/L aminopterin, and 16 μmol/L thymidine.