Table 1. Oligonucleotide Primers Used... | American Society of Hematology

Table 1.

Oligonucleotide Primers Used for RT-PCR Analysis of Fetal HSCs

Genes	5′ Primer	3′ Primer	Size^* (bp)
tal-1/SCl^†	TTGGGGAGCCGGATGCCTTC	CTCCCGGCTGTTGGTGAA	135
c-myb	ACAGCATATATAGCAGTGACG	AGCCTGAGCAAAACCCATCAA	659
GATA-2	CCCTAAGCAGCGCAGCAAGAC	TGACTTCTCCTGCATGCACT	439
GATA-3	ACCCCACTGTGGCGGCGAGAT	CACAGCACTAGAGACC	770
c-kit	TGTACTGCCAGTGGATGTGCA	TCGTCATCCTCCATGATGGCG	921
flk-1/KDR	CAACAAAGCGGAGAGGAG	ATGACGATGGACAAGTACCC	818
CD38	ATGGCCAACTGCGAGTTCAGC	GACTTTGGGGAAAAAGGCTTC	1,056
β-Actin	AACCGCGAGAAGATGACCCAG	TGCGCTCAGGAGGAGCAATGA	663

Genes	5′ Primer	3′ Primer	Size^* (bp)
tal-1/SCl^†	TTGGGGAGCCGGATGCCTTC	CTCCCGGCTGTTGGTGAA	135
c-myb	ACAGCATATATAGCAGTGACG	AGCCTGAGCAAAACCCATCAA	659
GATA-2	CCCTAAGCAGCGCAGCAAGAC	TGACTTCTCCTGCATGCACT	439
GATA-3	ACCCCACTGTGGCGGCGAGAT	CACAGCACTAGAGACC	770
c-kit	TGTACTGCCAGTGGATGTGCA	TCGTCATCCTCCATGATGGCG	921
flk-1/KDR	CAACAAAGCGGAGAGGAG	ATGACGATGGACAAGTACCC	818
CD38	ATGGCCAACTGCGAGTTCAGC	GACTTTGGGGAAAAAGGCTTC	1,056
β-Actin	AACCGCGAGAAGATGACCCAG	TGCGCTCAGGAGGAGCAATGA	663

Primers were selected to cross introns or checked for no amplification of same-size genomic products.

Predicted PCR product.

PCR was performed at a 1.5 mmol/L MgCl₂ final concentration and 58°C annealing temperature.