Primers Used for VH Gene Amplification
| Name . | Sequence (5′-3′) . |
|---|---|
| VH1 | AAATCGATACCACCATGGACTGGACCTGGAGG |
| VH1b | AAATCGATACCACCATGGACTGGACCTGGAG(C/A) |
| VH2a | AAATCGATACCACCATGGACACACTTTGCT(A/C)AC |
| VH2b | AAATCGATACCACCATGGACATACTTTGTTCCAC |
| VH3a | AAATCGATACCACCACCATGGAGTTTGGGCTGAGC |
| VH3b | AAATCGATACCACCACCATGGA(A/G)(C/T)T(G/T)(G/T)G(G/A)CT(G/C/T)(A/C/T)GC |
| VH4 | AAATCGATACCACCATGAAACACCTGTGGTTCTT |
| VH5 | AAATCGATACCACCATGGGGTCAACCGCCATC |
| VH6 | AAATCGATACCACCATGTCTGTCTCCTTCCTC |
| FR3 | GACACGGC(C/T)(C/G)T(G/A)TATTACTG |
| JH | GGACTAGTTTCTTACCTGAGGAGACGGTGACC |
| JHseq | ACCTGAGGAGACGGTGACC |
| Cμ | GGGTCGACGCAGGAGACGAGGGGGAAAAG |
| Cγ | GGGACTAGTAACTTTCTTGTCCACCTTGGTGTT |
| Cγ2 | AGACCGATGGGCCCTTGGTG |
| Cα | CGGGAAGACCTTGGGGCTG |
| Cδ | TGTCTGCACCCTGATATGATG |
| Name . | Sequence (5′-3′) . |
|---|---|
| VH1 | AAATCGATACCACCATGGACTGGACCTGGAGG |
| VH1b | AAATCGATACCACCATGGACTGGACCTGGAG(C/A) |
| VH2a | AAATCGATACCACCATGGACACACTTTGCT(A/C)AC |
| VH2b | AAATCGATACCACCATGGACATACTTTGTTCCAC |
| VH3a | AAATCGATACCACCACCATGGAGTTTGGGCTGAGC |
| VH3b | AAATCGATACCACCACCATGGA(A/G)(C/T)T(G/T)(G/T)G(G/A)CT(G/C/T)(A/C/T)GC |
| VH4 | AAATCGATACCACCATGAAACACCTGTGGTTCTT |
| VH5 | AAATCGATACCACCATGGGGTCAACCGCCATC |
| VH6 | AAATCGATACCACCATGTCTGTCTCCTTCCTC |
| FR3 | GACACGGC(C/T)(C/G)T(G/A)TATTACTG |
| JH | GGACTAGTTTCTTACCTGAGGAGACGGTGACC |
| JHseq | ACCTGAGGAGACGGTGACC |
| Cμ | GGGTCGACGCAGGAGACGAGGGGGAAAAG |
| Cγ | GGGACTAGTAACTTTCTTGTCCACCTTGGTGTT |
| Cγ2 | AGACCGATGGGCCCTTGGTG |
| Cα | CGGGAAGACCTTGGGGCTG |
| Cδ | TGTCTGCACCCTGATATGATG |