High-Affinity MoAb HB57 Can Prevent the Enhanced Degree of Ramos Apoptosis Induced by Intermediate-Affinity MoAb Mu53
| MoAb in Culture . | % Annexin-Positive Above Control . | |
|---|---|---|
| Final Concentration of Each MoAb (μg/mL) . | ||
| 100 . | 10 . | |
| MoAb HB57 | 14 ± 9 | 18 ± 4 |
| MoAb Mu53 | 52 ± 2 | 51 ± 5 |
| MoAb Mu53 + MoAb HB57 | 19 ± 3 | 34 ± 5 |
| MoAb in Culture . | % Annexin-Positive Above Control . | |
|---|---|---|
| Final Concentration of Each MoAb (μg/mL) . | ||
| 100 . | 10 . | |
| MoAb HB57 | 14 ± 9 | 18 ± 4 |
| MoAb Mu53 | 52 ± 2 | 51 ± 5 |
| MoAb Mu53 + MoAb HB57 | 19 ± 3 | 34 ± 5 |
The above data represent the mean ± SD from three separate experiments in which Ramos B cells were stained with FITC-annexin at t = 24, 28, and 36 hours of culture. The percentage of annexin-positive cells in control cultures containing medium alone was 15%, 27%, and 38%, respectively, for these pooled experiments. The MoAb:cell ratio at the concentration of 100 μg/mL was 20 μg/105 cells and at 10 μg/mL was 2 μg/105 cells.