Cellular number and composition in tissues of engrafted NOD/SCID/IL2rγnull mice
. | . | % nucleated cells (% hCD45+ cells) . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Injected cells, mice, and tissue type . | Total no. cells . | CD33 . | CD19 . | CD3 . | CD11c . | |||
| 1 × 105 Lin-CD34+ | ||||||||
| Mouse no. 1/ donor no. 1 | ||||||||
| BM | 2.4 × 107 | 8.2 (11.6) | 54.8 (77.6) | 10.7 (15.1) | 1.1 (1.6) | |||
| Spleen | 4.1 × 107 | 4.3 (6.4) | 33.5 (50.1) | 26.1 (39.1) | 2.2 (3.3) | |||
| Thymus | 3.1 × 105 | NE | 1.3 (1.3) | 96.2 (98.7) | NE | |||
| PB | NE | 4.0 (5.6) | 35.1 (49.3) | 19.8 (27.8) | NE | |||
| Mouse no. 2/donor no. 1 | ||||||||
| BM | 1.8 × 107 | 5.5 (6.8) | 56.5 (67.6) | 9.9 (12.2) | 2.9 (3.6) | |||
| Spleen | 3.2 × 107 | 2.1 (4.5) | 27.7 (58.8) | 15.9 (33.8) | 1.3 (2.8) | |||
| Thymus | 4.5 × 105 | NE | 1.1 (1.2) | 90.4 (98.8) | NE | |||
| PB | NE | 6.1 (7.5) | 53.8 (65.9) | 21.6 (40.1) | NE | |||
| Mouse no. 4/ donor no. 3 | ||||||||
| BM | 1.9 × 107 | 9.4 (11.3) | 52.9 (63.7) | 15.9 (19.1) | 0.62 (0.75) | |||
| Spleen | 4.4 × 107 | 3.5 (6.8) | 24.2 (47.4) | 20.8 (40.7) | 1.5 (2.9) | |||
| Thymus | 0.8 × 105 | NE | 0.88 (1.1) | 78.2 (98.9) | NE | |||
| PB | NE | 3.2 (4.7) | 61.3 (90.1) | 5.3 (7.8) | NE | |||
| Mouse no. 5/donor no. 3 | ||||||||
| BM | 2.1 × 107 | 10.2 (14.6) | 48.8 (82.0) | 9.4 (13.4) | 1.3 (1.9) | |||
| Spleen | 2.7 × 107 | 6.6 (11.4) | 30.4 (52.3) | 18.6 (32.0) | 1.1 (1.9) | |||
| Thymus | 1.1 × 105 | NE | 3.1 (3.7) | 81.1 (96.3) | NE | |||
| PB | NE | 9.8 (13.4) | 40.4 (55.1) | 16.8 (22.9) | NE | |||
| 2 × 104 Lin-CD34+CD38- | ||||||||
| Mouse no. 6/donor no. 4 | ||||||||
| BM | 2.6 × 107 | 3.1 (5.3) | 46.1 (78.7) | 8.1 (13.8) | 1.3 (2.2) | |||
| Spleen | 3.9v107 | 1.3 (2.7) | 40.2 (83.9) | 5.9 (12.3) | 0.54 (1.1) | |||
| Thymus | 1.9 × 105 | NE | 2.1 (2.3) | 89.4 (97.7) | NE | |||
| PB | NE | 5.4 (12.4) | 28.9 (66.3) | 9.3 (21.3) | NE | |||
| Mouse no. 7/donor no. 5 | ||||||||
| BM | 1.4 × 107 | 7.2 (14.2) | 39.6 (78.1) | 3.1 (6.1) | 0.82 (1.6) | |||
| Spleen | 2.2 × 107 | 2.4 (5.1) | 37.2 (79.3) | 6.8 (14.5) | 0.52 (1.1) | |||
| Thymus | 1.3 × 105 | NE | 0.6 (0.7) | 85.1 (99.2) | NE | |||
| PB | NE | 2.3 (41.7) | 49.3 (89.5) | 3.5 (6.4) | NE | |||
| Mouse no. 8/donor no. 6 | ||||||||
| BM | 1.1 × 107 | 6.1 (11.7) | 36.8 (70.6) | 7.7 (14.8) | 2.5 (4.8) | |||
| Spleen | 2.9 × 107 | 2.9 (4.6) | 33.8 (53.1) | 24.6 (38.6) | 2.4 (3.8) | |||
| Thymus | 1.9 × 105 | NE | 1.1 (1.2) | 94.1 (97.0) | NE | |||
| PB | NE | 8.1 (11.9) | 50.2 (73.5) | 10.0 (14.6) | NE | |||
. | . | % nucleated cells (% hCD45+ cells) . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Injected cells, mice, and tissue type . | Total no. cells . | CD33 . | CD19 . | CD3 . | CD11c . | |||
| 1 × 105 Lin-CD34+ | ||||||||
| Mouse no. 1/ donor no. 1 | ||||||||
| BM | 2.4 × 107 | 8.2 (11.6) | 54.8 (77.6) | 10.7 (15.1) | 1.1 (1.6) | |||
| Spleen | 4.1 × 107 | 4.3 (6.4) | 33.5 (50.1) | 26.1 (39.1) | 2.2 (3.3) | |||
| Thymus | 3.1 × 105 | NE | 1.3 (1.3) | 96.2 (98.7) | NE | |||
| PB | NE | 4.0 (5.6) | 35.1 (49.3) | 19.8 (27.8) | NE | |||
| Mouse no. 2/donor no. 1 | ||||||||
| BM | 1.8 × 107 | 5.5 (6.8) | 56.5 (67.6) | 9.9 (12.2) | 2.9 (3.6) | |||
| Spleen | 3.2 × 107 | 2.1 (4.5) | 27.7 (58.8) | 15.9 (33.8) | 1.3 (2.8) | |||
| Thymus | 4.5 × 105 | NE | 1.1 (1.2) | 90.4 (98.8) | NE | |||
| PB | NE | 6.1 (7.5) | 53.8 (65.9) | 21.6 (40.1) | NE | |||
| Mouse no. 4/ donor no. 3 | ||||||||
| BM | 1.9 × 107 | 9.4 (11.3) | 52.9 (63.7) | 15.9 (19.1) | 0.62 (0.75) | |||
| Spleen | 4.4 × 107 | 3.5 (6.8) | 24.2 (47.4) | 20.8 (40.7) | 1.5 (2.9) | |||
| Thymus | 0.8 × 105 | NE | 0.88 (1.1) | 78.2 (98.9) | NE | |||
| PB | NE | 3.2 (4.7) | 61.3 (90.1) | 5.3 (7.8) | NE | |||
| Mouse no. 5/donor no. 3 | ||||||||
| BM | 2.1 × 107 | 10.2 (14.6) | 48.8 (82.0) | 9.4 (13.4) | 1.3 (1.9) | |||
| Spleen | 2.7 × 107 | 6.6 (11.4) | 30.4 (52.3) | 18.6 (32.0) | 1.1 (1.9) | |||
| Thymus | 1.1 × 105 | NE | 3.1 (3.7) | 81.1 (96.3) | NE | |||
| PB | NE | 9.8 (13.4) | 40.4 (55.1) | 16.8 (22.9) | NE | |||
| 2 × 104 Lin-CD34+CD38- | ||||||||
| Mouse no. 6/donor no. 4 | ||||||||
| BM | 2.6 × 107 | 3.1 (5.3) | 46.1 (78.7) | 8.1 (13.8) | 1.3 (2.2) | |||
| Spleen | 3.9v107 | 1.3 (2.7) | 40.2 (83.9) | 5.9 (12.3) | 0.54 (1.1) | |||
| Thymus | 1.9 × 105 | NE | 2.1 (2.3) | 89.4 (97.7) | NE | |||
| PB | NE | 5.4 (12.4) | 28.9 (66.3) | 9.3 (21.3) | NE | |||
| Mouse no. 7/donor no. 5 | ||||||||
| BM | 1.4 × 107 | 7.2 (14.2) | 39.6 (78.1) | 3.1 (6.1) | 0.82 (1.6) | |||
| Spleen | 2.2 × 107 | 2.4 (5.1) | 37.2 (79.3) | 6.8 (14.5) | 0.52 (1.1) | |||
| Thymus | 1.3 × 105 | NE | 0.6 (0.7) | 85.1 (99.2) | NE | |||
| PB | NE | 2.3 (41.7) | 49.3 (89.5) | 3.5 (6.4) | NE | |||
| Mouse no. 8/donor no. 6 | ||||||||
| BM | 1.1 × 107 | 6.1 (11.7) | 36.8 (70.6) | 7.7 (14.8) | 2.5 (4.8) | |||
| Spleen | 2.9 × 107 | 2.9 (4.6) | 33.8 (53.1) | 24.6 (38.6) | 2.4 (3.8) | |||
| Thymus | 1.9 × 105 | NE | 1.1 (1.2) | 94.1 (97.0) | NE | |||
| PB | NE | 8.1 (11.9) | 50.2 (73.5) | 10.0 (14.6) | NE | |||
BM, spleen, and thymus were harvested from engrafted NOD/SCID/IL2rγnull mice at 3 months after transplantation. Total cell numbers in BM and thymus represent the cells harvested from 2 femurs for BM and those harvested from a hemilobe for thymus. Recipients 1, 2, 4, and 5 received transplants of 1 × 105 Lin-CD34+ cells. Recipients 6, 7, and 8 received transplants of 2 × 104 Lin-CD34+CD38- cells. NE indicates not examined.