Chimerism of human CD45+ cells in NOD/SCID/β2mnull mice and NOD/SCID/IL2rynull mice
. | % nucleated cells . | . | . | ||
|---|---|---|---|---|---|
| Mouse no. (donor no.) . | PB . | BM . | Spleen . | ||
| NOD/SCID/IL2rγnull | |||||
| 1 (1) | 71.2 | 70.9 | 66.8 | ||
| 2 (1) | 81.7 | 81.4 | 47.1 | ||
| 3 (2) | 50.1 | 58.8 | 49.5 | ||
| 4 (3) | 68.0 | 83.1 | 51.1 | ||
| 5 (3) | 73.3 | 70.1 | 58.1 | ||
| Mean ± SD | 68.9 ± 11.6* | 72.9 ± 9.8* | 54.5 ± 8.0* | ||
| NOD/SCID/β2mnull | |||||
| 1 (1) | 10.4 | 46.1 | 22.0 | ||
| 2 (2) | 11.6 | 31.5 | 24.3 | ||
| 3 (3) | 6.9 | 18.1 | 20.7 | ||
| 4 (3) | 20.7 | 30.4 | 31.2 | ||
| Mean ± SD | 12.4 ± 5.9* | 31.5 ± 11.5* | 22.6 ± 4.7* | ||
. | % nucleated cells . | . | . | ||
|---|---|---|---|---|---|
| Mouse no. (donor no.) . | PB . | BM . | Spleen . | ||
| NOD/SCID/IL2rγnull | |||||
| 1 (1) | 71.2 | 70.9 | 66.8 | ||
| 2 (1) | 81.7 | 81.4 | 47.1 | ||
| 3 (2) | 50.1 | 58.8 | 49.5 | ||
| 4 (3) | 68.0 | 83.1 | 51.1 | ||
| 5 (3) | 73.3 | 70.1 | 58.1 | ||
| Mean ± SD | 68.9 ± 11.6* | 72.9 ± 9.8* | 54.5 ± 8.0* | ||
| NOD/SCID/β2mnull | |||||
| 1 (1) | 10.4 | 46.1 | 22.0 | ||
| 2 (2) | 11.6 | 31.5 | 24.3 | ||
| 3 (3) | 6.9 | 18.1 | 20.7 | ||
| 4 (3) | 20.7 | 30.4 | 31.2 | ||
| Mean ± SD | 12.4 ± 5.9* | 31.5 ± 11.5* | 22.6 ± 4.7* | ||
To compare the engraftment levels in the two strains, 1 × 105 Lin- CD34+ cells derived from 3 CB samples were transplanted into 5 NOD/SCID/IL2rγnull mice and 4 NOD/SCID/β2mnull mice. At 3 months after transplantation, BM, spleen, and peripheral blood (PB) of the recipient mice were analyzed for the engraftment of human cells. Data show percentages of human CD45+ cells in each tissue.
P < .05.