Table 1. Primers used in retroviral... | American Society of Hematology

Table 1.

Primers used in retroviral vector construction

No.	Primers	Sequences
1	5′ wtNKG2D	GCGAATTCGCCACCATGGCATTGATTCGTGATCGA
2	3′ wtNKG2D	GGCGCTCGAGTTACACCGCCCTTTTCATGCAGAT
3	5′ chNKG2D	GGCGAATTCGCATTGATTCGTGATCGAAAGTCT
4	5′ wtDap10	GCAAGTCGACGCCACCATGGACCCCCCAGGCTACC
5	3′ wtDap10	GGCGAATTCTCAGCCTCTGCCAGGCATGTTGAT
6	3′ chDap10	GGCAGAATTCGCCTCTGCCAGGCATGTTGATGTA
7	5′ wtDap12	GTTAGAATTCGCCACCATGGGGGCTCTGGAGCCCT
8	3′ wtDap12	GCAACTCGAGTCATCTGTAATATTGCCTCTGTG
9	5′ ATG-CD3ζ	GGCGTCGACACCATGAGAGCAAAATTCAGCAGGAG
10	3′ ATG-CD3ζ	GCTTGAATTCGCGAGGGGCCAGGGTCTGCATAT
11	5′ CD3ζ-TAA	GCAGAATTCAGAGCAAAATTCAGCAGGAGTGC
12	3′ CD3ζ-TAA	GCTTTCTCGAGTTAGCGAGGGGCCAGGGTCTGCAT
13	5′ Rae-1	GCATGTCGACGCCACCATGGCCAAGGCAGCAGTGA
14	3′ Rae-1	GCGGCTCGAGTCACATCGCAAATGCAAATGC
15	5′ H60	GTTAGAATTCGCCACCATGGCAAAGGGAGCCACC
16	3′ H60	GCGCTCGAGTCATTTTTTCTTCAGCATACACCAAG

No.	Primers	Sequences
1	5′ wtNKG2D	GCGAATTCGCCACCATGGCATTGATTCGTGATCGA
2	3′ wtNKG2D	GGCGCTCGAGTTACACCGCCCTTTTCATGCAGAT
3	5′ chNKG2D	GGCGAATTCGCATTGATTCGTGATCGAAAGTCT
4	5′ wtDap10	GCAAGTCGACGCCACCATGGACCCCCCAGGCTACC
5	3′ wtDap10	GGCGAATTCTCAGCCTCTGCCAGGCATGTTGAT
6	3′ chDap10	GGCAGAATTCGCCTCTGCCAGGCATGTTGATGTA
7	5′ wtDap12	GTTAGAATTCGCCACCATGGGGGCTCTGGAGCCCT
8	3′ wtDap12	GCAACTCGAGTCATCTGTAATATTGCCTCTGTG
9	5′ ATG-CD3ζ	GGCGTCGACACCATGAGAGCAAAATTCAGCAGGAG
10	3′ ATG-CD3ζ	GCTTGAATTCGCGAGGGGCCAGGGTCTGCATAT
11	5′ CD3ζ-TAA	GCAGAATTCAGAGCAAAATTCAGCAGGAGTGC
12	3′ CD3ζ-TAA	GCTTTCTCGAGTTAGCGAGGGGCCAGGGTCTGCAT
13	5′ Rae-1	GCATGTCGACGCCACCATGGCCAAGGCAGCAGTGA
14	3′ Rae-1	GCGGCTCGAGTCACATCGCAAATGCAAATGC
15	5′ H60	GTTAGAATTCGCCACCATGGCAAAGGGAGCCACC
16	3′ H60	GCGCTCGAGTCATTTTTTCTTCAGCATACACCAAG

For facilitated cloning, restriction sites were inserted into primers (underlined). Optimal Kozak sequences (in italics) were added to the 5′ primers of genes.