Table 1. Surface phenotype of liver and... | American Society of Hematology

Table 1.

Surface phenotype of liver and bone marrow SP and non-SP cells

	Liver, %
	SP tip	SP shoulder	Non-SP	SP	Non-SP
Sca-1⁺	60	30	15	90	5
CD45⁺	3	64	32	99	95
c-kit⁺*	< 1	1	40	95	12
Lin⁺†	2	4	10	10	85
CD31⁺	10	43	50	95	50
CD29⁺	99	90	70	95	25
CD49f⁺	90	20	50	90	5

	Liver, %
	SP tip	SP shoulder	Non-SP	SP	Non-SP
Sca-1⁺	60	30	15	90	5
CD45⁺	3	64	32	99	95
c-kit⁺*	< 1	1	40	95	12
Lin⁺†	2	4	10	10	85
CD31⁺	10	43	50	95	50
CD29⁺	99	90	70	95	25
CD49f⁺	90	20	50	90	5

FACS-based analysis of surface immunostaining was used to determine the average percentage of cells expressing a specified surface marker in each cell population analyzed.

Enzyme digestion diminishes the intensity of c-kit immunostaining as detailed in “CD45⁺ SP tip cells possess phenotypic characteristics distinct from bone marrow HSCs.”

†

Lin indicates CD3, B220, CD11b, GR-1, and Ter-119.

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