Direct recognition and lysis of leukemia cells by NIK-1: experiment 2
Target cells . | % cytotoxicity, at 10:1 E/T ratio . |
|---|---|
| C2F8 cells | |
| No anti-HLA mAb | 27.6 |
| With anti-HLA-DR mAb | 28.0 |
| With anti-HLA-DQ mAb | 30.3 |
| With anti-HLA-DP mAb | 3.1 |
| Freshly isolated leukemia cells | |
| From donor no. 1 (AML M1) | |
| No anti-HLA mAb | 45.1 |
| With anti-HLA-DR mAb | 43.2 |
| With anti-HLA-DQ mAb | 41.6 |
| With anti-HLA-DP mAb | 5.6 |
| From donor no. 2 (AML M2) | |
| No anti-HLA mAb | 33.6 |
| With anti-HLA-DR mAb | 31.7 |
| With anti-HLA-DQ mAb | 35.1 |
| With anti-HLA-DP mAb | 5.6 |
Target cells . | % cytotoxicity, at 10:1 E/T ratio . |
|---|---|
| C2F8 cells | |
| No anti-HLA mAb | 27.6 |
| With anti-HLA-DR mAb | 28.0 |
| With anti-HLA-DQ mAb | 30.3 |
| With anti-HLA-DP mAb | 3.1 |
| Freshly isolated leukemia cells | |
| From donor no. 1 (AML M1) | |
| No anti-HLA mAb | 45.1 |
| With anti-HLA-DR mAb | 43.2 |
| With anti-HLA-DQ mAb | 41.6 |
| With anti-HLA-DP mAb | 5.6 |
| From donor no. 2 (AML M2) | |
| No anti-HLA mAb | 33.6 |
| With anti-HLA-DR mAb | 31.7 |
| With anti-HLA-DQ mAb | 35.1 |
| With anti-HLA-DP mAb | 5.6 |
Cytotoxicity of NIK-1 against leukemia cell line and freshly isolated leukemia cells in the presence or absence of anti-HLA-DR, anti-HLA-DQ, or anti-HLA-DP mAb.
E/T ratio indicates effector-target ratio.