Direct recognition and lysis of leukemia cells by NIK-1: experiment 1
. | WT1 expression level . | HLA-DP5 expression . | % cytotoxicity, at E/T ratio . | . | . | ||
|---|---|---|---|---|---|---|---|
| Target cells . | . | . | 20:1 ratio . | 10:1 ratio . | 5:1 ratio . | ||
| Cell lines | |||||||
| C2F8; leukemia | 2.3 × 100 | + | 38.8 | 34.4 | 31.2 | ||
| MEG01; leukemia | 8.4 × 10−1 | − | 3.6 | 1.8 | 1.2 | ||
| KAZZ; leukemia | 1.1 × 100 | − | 2.1 | 2.7 | 0.9 | ||
| IZU; lymphoma | 1.1 × 10−5 | + | 1.5 | 0.5 | 1.0 | ||
| IKE; lymphoma | 8.6 × 10−5 | + | 0.7 | 1.8 | 0.5 | ||
| Daudi; lymphoma | 3.8 × 10−5 | − | 2.0 | 0.3 | 0.4 | ||
| Freshly isolated leukemia cells | |||||||
| From donor no. 1 (AML M1) | 3.5 × 10−1 | + | 45.1 | 34.2 | 21.0 | ||
| From donor no. 2 (AML M2) | 2.7 × 10−1 | + | 33.6 | 24.3 | 18.9 | ||
| From donor no. 3 (AML M2) | 8.6 × 10−2 | + | 31.2 | 18.9 | 13.1 | ||
| From donor no. 4 (AML M1) | 6.5 × 10−1 | − | 2.7 | 2.1 | 0.6 | ||
| From donor no. 5 (AML M2) | 7.2 × 10−1 | − | 1.4 | 1.8 | 1.0 | ||
| From donor no. 6 (ALL L2) | 5.8 × 10−1 | − | 0.0 | 0.3 | 0.0 | ||
. | WT1 expression level . | HLA-DP5 expression . | % cytotoxicity, at E/T ratio . | . | . | ||
|---|---|---|---|---|---|---|---|
| Target cells . | . | . | 20:1 ratio . | 10:1 ratio . | 5:1 ratio . | ||
| Cell lines | |||||||
| C2F8; leukemia | 2.3 × 100 | + | 38.8 | 34.4 | 31.2 | ||
| MEG01; leukemia | 8.4 × 10−1 | − | 3.6 | 1.8 | 1.2 | ||
| KAZZ; leukemia | 1.1 × 100 | − | 2.1 | 2.7 | 0.9 | ||
| IZU; lymphoma | 1.1 × 10−5 | + | 1.5 | 0.5 | 1.0 | ||
| IKE; lymphoma | 8.6 × 10−5 | + | 0.7 | 1.8 | 0.5 | ||
| Daudi; lymphoma | 3.8 × 10−5 | − | 2.0 | 0.3 | 0.4 | ||
| Freshly isolated leukemia cells | |||||||
| From donor no. 1 (AML M1) | 3.5 × 10−1 | + | 45.1 | 34.2 | 21.0 | ||
| From donor no. 2 (AML M2) | 2.7 × 10−1 | + | 33.6 | 24.3 | 18.9 | ||
| From donor no. 3 (AML M2) | 8.6 × 10−2 | + | 31.2 | 18.9 | 13.1 | ||
| From donor no. 4 (AML M1) | 6.5 × 10−1 | − | 2.7 | 2.1 | 0.6 | ||
| From donor no. 5 (AML M2) | 7.2 × 10−1 | − | 1.4 | 1.8 | 1.0 | ||
| From donor no. 6 (ALL L2) | 5.8 × 10−1 | − | 0.0 | 0.3 | 0.0 | ||
Cytotoxicity of NIK-1 against various cell lines and freshly isolated leukemia cells. E/T ratio indicates effector-target ratio.