WT1-specific and HLA-DP5-restricted cytotoxicity by NIK-1: experiment 1
. | % cytotoxicity, at E/T ratio . | . | . | ||
|---|---|---|---|---|---|
| Target cells . | 10:1 ratio . | 5:1 ratio . | 2.5:1 ratio . | ||
| Auto LCL (HLA-DP5-positive) | |||||
| Without WT1 peptide | 10.3 | 6.3 | 3.1 | ||
| With WT1 peptide | 70.2 | 63.2 | 49.1 | ||
| Allo LCL no. 1 (HLA-DP5-positive) | |||||
| Without WT1 peptide | 8.9 | 7.3 | 3.4 | ||
| With WT1 peptide | 73.9 | 62.1 | 50.1 | ||
| Allo LCL no. 2 (HLA-DP5-positive) | |||||
| Without WT1 peptide | 5.3 | 5.1 | 4.1 | ||
| With WT1 peptide | 63.2 | 56.1 | 47.3 | ||
| Allo LCL no. 3 (HLA-DP5-negative) | |||||
| Without WT1 peptide | 1.0 | 0.5 | 0.0 | ||
| With WT1 peptide | 2.1 | 0.8 | 0.1 | ||
| Allo LCL no. 4 (HLA-DP5-negative) | |||||
| Without WT1 peptide | 2.1 | 0.0 | 0.1 | ||
| With WT1 peptide | 4.1 | 0.8 | 0.0 | ||
. | % cytotoxicity, at E/T ratio . | . | . | ||
|---|---|---|---|---|---|
| Target cells . | 10:1 ratio . | 5:1 ratio . | 2.5:1 ratio . | ||
| Auto LCL (HLA-DP5-positive) | |||||
| Without WT1 peptide | 10.3 | 6.3 | 3.1 | ||
| With WT1 peptide | 70.2 | 63.2 | 49.1 | ||
| Allo LCL no. 1 (HLA-DP5-positive) | |||||
| Without WT1 peptide | 8.9 | 7.3 | 3.4 | ||
| With WT1 peptide | 73.9 | 62.1 | 50.1 | ||
| Allo LCL no. 2 (HLA-DP5-positive) | |||||
| Without WT1 peptide | 5.3 | 5.1 | 4.1 | ||
| With WT1 peptide | 63.2 | 56.1 | 47.3 | ||
| Allo LCL no. 3 (HLA-DP5-negative) | |||||
| Without WT1 peptide | 1.0 | 0.5 | 0.0 | ||
| With WT1 peptide | 2.1 | 0.8 | 0.1 | ||
| Allo LCL no. 4 (HLA-DP5-negative) | |||||
| Without WT1 peptide | 2.1 | 0.0 | 0.1 | ||
| With WT1 peptide | 4.1 | 0.8 | 0.0 | ||
Cytotoxicity of NIK-1 against autologous (auto) and various allogeneic (allo) LCL loaded or unloaded with WT1 peptide. E/T ratio indicates effector-target ratio.