Determination of the number of intravenously transplanted old CRUs that home to the BM of young irradiated mice
. | Negative mice . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Cell type injected . | 5 wk . | 10 wk . | 17 wk . | 26 wk . | |||
| Fresh old BM* | |||||||
| 2 × 103 | 12 of 15 | 12 of 15 | 11 of 14 | 11 of 14 | |||
| 6 × 103 | 5 of 14 | 5 of 14 | 8 of 14 | 8 of 14 | |||
| 2 × 104 | 1 of 14 | 1 of 14 | 2 of 14 | 3 of 14 | |||
| 6 × 104 | 0 of 8 | 0 of 8 | 0 of 8 | 0 of 8 | |||
| 1/CRU frequency (±SEM) | 7006 (5548 ∼ 8848) | 7006 (5548 ∼ 8848) | 10 019 (7923 ∼ 12 671) | 11 304 (8932 ∼ 14 306) | |||
| No. CRUs injected (±SEM) | 428 (339 ∼ 541) | 428 (339 ∼ 541) | 299 (237 ∼ 379) | 265 (210 ∼ 336) | |||
| Homed old BM* | |||||||
| 0.005 | 6 of 6 | 6 of 6 | 6 of 6 | 6 of 6 | |||
| 0.014 | 10 of 12 | 8 of 9 | 7 of 8 | 4 of 5 | |||
| 0.042 | 9 of 14 | 8 of 13 | 8 of 13 | 9 of 13 | |||
| 0.125 | 4 of 10 | 4 of 10 | 4 of 10 | 4 of 10 | |||
| CRU frequency, 1 per primary BM fraction (±SEM) | 0.113 (0.085 ∼ 0.151) | 0.116 (0.086 ∼ 0.157) | 0.115 (0.085 ∼ 0.155) | 0.124 (0.091 ∼ 0.169) | |||
| 1/CRU frequency, no. CRUs recovered per primary BM (±SEM) | 8.8 (6.6 ∼ 11.8) | 8.6 (6.4 ∼ 11.6) | 8.7 (6.5 ∼ 11.8) | 8.1 (5.9 ∼ 11) | |||
. | Negative mice . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Cell type injected . | 5 wk . | 10 wk . | 17 wk . | 26 wk . | |||
| Fresh old BM* | |||||||
| 2 × 103 | 12 of 15 | 12 of 15 | 11 of 14 | 11 of 14 | |||
| 6 × 103 | 5 of 14 | 5 of 14 | 8 of 14 | 8 of 14 | |||
| 2 × 104 | 1 of 14 | 1 of 14 | 2 of 14 | 3 of 14 | |||
| 6 × 104 | 0 of 8 | 0 of 8 | 0 of 8 | 0 of 8 | |||
| 1/CRU frequency (±SEM) | 7006 (5548 ∼ 8848) | 7006 (5548 ∼ 8848) | 10 019 (7923 ∼ 12 671) | 11 304 (8932 ∼ 14 306) | |||
| No. CRUs injected (±SEM) | 428 (339 ∼ 541) | 428 (339 ∼ 541) | 299 (237 ∼ 379) | 265 (210 ∼ 336) | |||
| Homed old BM* | |||||||
| 0.005 | 6 of 6 | 6 of 6 | 6 of 6 | 6 of 6 | |||
| 0.014 | 10 of 12 | 8 of 9 | 7 of 8 | 4 of 5 | |||
| 0.042 | 9 of 14 | 8 of 13 | 8 of 13 | 9 of 13 | |||
| 0.125 | 4 of 10 | 4 of 10 | 4 of 10 | 4 of 10 | |||
| CRU frequency, 1 per primary BM fraction (±SEM) | 0.113 (0.085 ∼ 0.151) | 0.116 (0.086 ∼ 0.157) | 0.115 (0.085 ∼ 0.155) | 0.124 (0.091 ∼ 0.169) | |||
| 1/CRU frequency, no. CRUs recovered per primary BM (±SEM) | 8.8 (6.6 ∼ 11.8) | 8.6 (6.4 ∼ 11.6) | 8.7 (6.5 ∼ 11.8) | 8.1 (5.9 ∼ 11) | |||
Experimental design and determination of the frequencies and absolute numbers of CRUs transplanted and recovered after homing are the same as described in Table 1, except that donor cells were harvested initially from old (20-22 months of age) mice and that young (6-8 weeks of age) mice were used as recipients. For negative mice, values for fresh young BM and homed young BM are proportions.
No. fresh cells or fraction of homed primary BM cells injected per mouse.